ArticleThe Journal of cell biology1985
Polymerization of tubulin in vivo: direct evidence for assembly onto microtubule ends and from centrosomes.
Article in The Journal of cell biology, 1985. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
What it found
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Who cites it
57 citing papers in PubMed, 144 citations in OpenAlex.
- Centrocortin potentiates co-translational localization of its mRNA to the centrosome via dynein.bioRxiv : the preprint server for biology · 2024Article
- Triple labelling of actin filaments, intermediate filaments and microtubules for broad application in cell biology: uncovering the cytoskeletal composition in tunneling nanotubes.Histochemistry and cell biology · 2019Article
- In focus in HCB.Histochemistry and cell biology · 2019Article
- Helical organization of microtubules occurs in a minority of tunneling membrane nanotubes in normal and cancer urothelial cells.Scientific reports · 2018Article
- A toolkit for studying cellular reorganization during early embryogenesis in Arabidopsis thaliana.The Plant journal : for cell and molecular biology · 2018Article
- Cytoskeletal Network Morphology Regulates Intracellular Transport Dynamics.Biophysical journal · 2015Article
- What determines cell size?BMC biology · 2012Article
- Article
- Cruising along microtubule highways: how membranes move through the secretory pathway.The Journal of cell biology · 1998Review
- Actomyosin-based retrograde flow of microtubules in the lamella of migrating epithelial cells influences microtubule dynamic instability and turnover and is associated with microtubule breakage and treadmilling.The Journal of cell biology · 1997Article
- Centrosomes isolated from Spisula solidissima oocytes contain rings and an unusual stoichiometric ratio of alpha/beta tubulin.The Journal of cell biology · 1997Article
- Removal of MAP4 from microtubules in vivo produces no observable phenotype at the cellular level.The Journal of cell biology · 1996Article
- Expression of fibronectin, laminin and ribosomes in normal and nocodazole-treated neonatal heart cells in culture: a study by laser scanning confocal microscopy and immunocytochemistry.Cell and tissue research · 1995Article
- Microtubule dynamics in fish melanophores.The Journal of cell biology · 1994Article
- Intrinsic microtubule stability in interphase cells.The Journal of cell biology · 1994Article
- Identification and molecular characterization of E-MAP-115, a novel microtubule-associated protein predominantly expressed in epithelial cells.The Journal of cell biology · 1993Article
- Do photobleached fluorescent microtubules move?: re-evaluation of fluorescence laser photobleaching both in vitro and in growing Xenopus axon.The Journal of cell biology · 1993Article
- Okadaic acid induces interphase to mitotic-like microtubule dynamic instability by inactivating rescue.The Journal of cell biology · 1992Article
- Newly assembled microtubules are concentrated in the proximal and distal regions of growing axons.The Journal of cell biology · 1992Article
- Steady-state kinetic mechanism of bovine brain tubulin: tyrosine ligase.The Biochemical journal · 1992Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Microtubule assembly in vivo was studied by hapten-mediated immunocytochemistry. Tubulin was derivatized with dichlorotriazinylaminofluorescein (DTAF) and microinjected into living, interphase mammalian cells. Sites of incorporation were determined at the level of individual microtubules by double-label immunofluorescence. The haptenized tubulin was localized by an anti-fluorescein antibody and a second antibody conjugated with fluorescein. Total microtubules were identified by anti-tubulin and a secondary antibody conjugated with rhodamine. Contrary to recent studies (Salmon, E. D., et al., 1984, J. Cell Biol., 99:2165-2174; Saxton, W. M., et al., 1984, J. Cell Biol., 99:2175-2186) which suggest that tubulin incorporates all along the length of microtubules in vivo, we found that microtubule assembly in interphase cells was in vivo, as in vitro, an end-mediated process. Microtubules that radiated out toward the cell periphery incorporated the DTAF-tubulin solely at their distal, that is, their plus ends. We also found that a proportion of the microtubules connected to the centrosomes incorporated the DTAF-tubulin along their entire length, which suggests that the centrosome can nucleate the formation of new microtubules.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.