Evidence map›Paper›PMID 38866007›Full record

ArticleCell reports methods2024

Development of an efficient, effective, and economical technology for proteome analysis.

Katherine R Martin, Ha T Le, Ahmed Abdelgawad, Canyuan Yang, Guotao Lu, Jessica L Keffer, Xiaohui Zhang, Zhihao Zhuang, Papa Nii Asare-Okai, Clara S Chan and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports methods, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Article
  5. FTO separation-of-function mutations alter mbioRxiv : the preprint server for biology · 2026
    Article
  6. Article
  7. Article
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  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Applied and environmental microbiology · 2025
    Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors.

Katherine R MartinDepartment of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA.
Ha T LeDepartment of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA.
Ahmed AbdelgawadDepartment of Biological Sciences, University of Delaware, Newark, DE 19716, USA; Department of Medical and Molecular Sciences, University of Delaware, Newark, DE 19716, USA.
Canyuan YangDepartment of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA.
Guotao LuCDS Analytical, LLC, Oxford, PA 19363, USA.
Jessica L KefferDepartment of Earth Sciences, University of Delaware, Newark, DE 19716, USA.
Xiaohui ZhangCDS Analytical, LLC, Oxford, PA 19363, USA.
Zhihao ZhuangDepartment of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA.
Papa Nii Asare-OkaiDepartment of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA.
Clara S ChanDepartment of Earth Sciences, University of Delaware, Newark, DE 19716, USA; School of Marine Science and Policy, University of Delaware, Newark, DE 19716, USA.
Mona BatishDepartment of Biological Sciences, University of Delaware, Newark, DE 19716, USA; Department of Medical and Molecular Sciences, University of Delaware, Newark, DE 19716, USA. Electronic address: batish@udel.edu.
Yanbao YuDepartment of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA. Electronic address: yybyu@udel.edu.

Funding

This renovation project will create over 1455 sq. ft. of state- of-the-art reseaP20GM104316 · NIGMS · UNIVERSITY OF DELAWARE · PI FOX, JOSEPH M · 2014 to 2024
$26.8M
NIH ADMINISTRATIVE SUPPLEMENT AUTOMATED PEPTIDE SYNTHESIZER ZHUANGR01GM129468 · NIGMS · UNIVERSITY OF DELAWARE · PI ZHUANG, ZHIHAO · 2019 to 2022
$1.3M
NIGMS NIH HHS P20 GM104316NIGMS NIH HHS R01 GM129468
6 · The paper itself

Abstract

We present an efficient, effective, and economical approach, named E3technology, for proteomics sample preparation. By immobilizing silica microparticles into the polytetrafluoroethylene matrix, we develop a robust membrane medium, which could serve as a reliable platform to generate proteomics-friendly samples in a rapid and low-cost fashion. We benchmark its performance using different formats and demonstrate them with a variety of sample types of varied complexity, quantity, and volume. Our data suggest that E3technology provides proteome-wide identification and quantitation performance equivalent or superior to many existing methods. We further propose an enhanced single-vessel approach, named E4technology, which performs on-filter in-cell digestion with minimal sample loss and high sensitivity, enabling low-input and low-cell proteomics. Lastly, we utilized the above technologies to investigate RNA-binding proteins and profile the intact bacterial cell proteome.

Indexed as

ProteomeProteomicsPolytetrafluoroethyleneSilicon DioxidePolytetrafluoroethyleneProteomeSilicon DioxideCP: BiotechnologyE3technologyE4technologyEmpore membraneglass beadsin-cell digestionon-filter digestionproteomicssample preparationsilica microparticles

Identifiers

PMID38866007
PMCPMC11228373

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.