Evidence map›Paper›PMID 38861179›Full record

ArticlePlanta2024

Identification and functional analysis of floral terpene synthase genes in Curcuma alismatifolia.

Zihan Cao, Lan Wang, Di Huang, Guilan Wu, Xinyue Li, Yuechong Yue, Yunyi Yu, Rangcai Yu, Yanping Fan

Abstract read
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In one paragraph

Article in Planta, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Zihan Cao *The Research Center for Ornamental Plants, College of Horticulture, South China Agricultural University, Guangzhou, 510642, China.
Lan Wang *The Research Center for Ornamental Plants, College of Horticulture, South China Agricultural University, Guangzhou, 510642, China.
Di HuangThe Research Center for Ornamental Plants, College of Horticulture, South China Agricultural University, Guangzhou, 510642, China.
Guilan WuCollege of Life Sciences, South China Agricultural University, Guangzhou, 510642, China.
Xinyue LiThe Research Center for Ornamental Plants, College of Horticulture, South China Agricultural University, Guangzhou, 510642, China.
Yuechong YueThe Research Center for Ornamental Plants, College of Horticulture, South China Agricultural University, Guangzhou, 510642, China.
Yunyi YuThe Research Center for Ornamental Plants, College of Horticulture, South China Agricultural University, Guangzhou, 510642, China.
Rangcai YuCollege of Life Sciences, South China Agricultural University, Guangzhou, 510642, China.
Yanping FanThe Research Center for Ornamental Plants, College of Horticulture, South China Agricultural University, Guangzhou, 510642, China. fanyanping@scau.edu.cn.

Funding

Key-Areas Research and Development Program of Guangdong Province 2020B020220007
6 · The paper itself

Abstract

MAIN

conclusionCaTPS2 and CaTPS3 were significantly expressed in flowers of Curcuma alismatifolia 'Shadow' and demonstrated bifunctional enzyme activity, CaTPS2 generated linalool and nerolidol as products, and CaTPS3 catalyzed β-myrcene and β-farnesene formation. This study presents the discovery and functional characterization of floral terpene synthase (TPS) genes in Curcuma alismatifolia 'Shadow', a cultivar renowned for its unique fragrance. Addressing the gap in understanding the genetic basis of floral scent in this species, we identified eight TPS genes through comprehensive transcriptome sequencing. Among these, CaTPS2 and CaTPS3 were significantly expressed in floral tissues and demonstrated bifunctional enzyme activity corresponding to the major volatile compounds detected in 'Shadow'. Functional analyses, including in vitro assays complemented with rigorous controls and alternative identification methods, elucidated the roles of these TPS genes in terpenoid biosynthesis. In vitro studies were conducted via heterologous expression in E. coli, followed by purification of the recombinant protein using affinity chromatography, enzyme assays were performed with GPP/FPP as the substrate, and volatile products were inserted into the GC-MS for analysis. Partially purified recombinant protein of CaTPS2 catalyzed GPP and FPP to produce linalool and nerolidol, respectively, while partially purified recombinant protein of CaTPS3 generated β-myrcene and β-farnesene with GPP and FPP as substrates, respectively. Real-time quantitative PCR further validated the expression patterns of these genes, correlating with terpenoid accumulation in different plant tissues. Our findings illuminate the molecular mechanisms underpinning floral fragrance in C. alismatifolia and provide a foundation for future genetic enhancements of floral scent in ornamental plants. This study, therefore, contributes to the broader understanding of terpenoid biosynthesis in plant fragrances, paving the way for biotechnological applications in horticulture plant breeding.

Indexed as

Acyclic MonoterpenesAlkyl and Aryl TransferasesCurcumaFlowersSesquiterpenesGene Expression Regulation, PlantOdorantsPhylogenyPlant ProteinsTerpenesVolatile Organic CompoundsAcyclic MonoterpenesAlkyl and Aryl Transferasesbeta-farnesenebeta-myrcenelinaloolnerolidolPlant ProteinsSesquiterpenesTerpenesterpene synthaseVolatile Organic CompoundsBiosynthesisCurcuma alismatifoliaFloral scentTerpene synthaseTerpenoidsVolatile emission

Identifiers

PMID38861179

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.