High Throughput Glycerate Kinase Activity Assay Using Crude Leaf Extract and Recombinant Enzyme to Determine Kinetic Parameters K

Ludmila V Roze, Audrey Johnson, Berkley J Walker

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Ludmila V RozeDepartment of Energy Plant Research Laboratory, Michigan State University, East Lansing, MI, USA.

Audrey JohnsonDepartment of Energy Plant Research Laboratory, Michigan State University, East Lansing, MI, USA.

Berkley J WalkerDepartment of Energy Plant Research Laboratory, Michigan State University, East Lansing, MI, USA. Berkley@msu.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We describe an assay for measuring the activity of D-glycerate 3-kinase (GLYK) in a 96-well microplate format with the use of a set of coupling enzymes. The assay is appropriate for use with a crude protein extract prepared from leaf tissue and with the recombinant purified enzyme. The 96-well microplate format reduces the needed amounts of reagents and coupling enzymes, making the assay less expensive, high throughput, and suitable for the determination of kinetic parameters K

Indexed as

Enzyme AssaysPlant ExtractsPlant LeavesRecombinant ProteinsHigh-Throughput Screening AssaysKineticsPlant ExtractsRecombinant Proteins3-PhosphoglycerateD-Glycerate 3-kinasePhotorespirationRecombinant D-glycerate 3-kinase

Identifiers

PMID38861080

DOI10.1016/0031-9422(88)80176-6

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.