ArticleNature chemical biology2024
Optimizing multicopy chromosomal integration for stable high-performing strains.
Article in Nature chemical biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Engineering nonconventional yeasts to produce carboxylic acids: Advances and Prospects.Synthetic and systems biotechnology · 2027Review
- A hybrid system enables plasmid copy number control in yeast.Nature communications · 2026Article
- Integrating microbial cell factory with new-to-nature photobiocatalysis for de novo biosynthesis of D-homotryptophan.Nature communications · 2026Article
- Combining computer-aided enzyme design and chromosomal integration for plasmid-free biosynthesis of 1,5-pentanediol in Escherichia coli.Microbial cell factories · 2026Article
- Transposon engineering ofEngineering in life sciences · 2026Article
- Push-pull-block framework for lycopene biosynthesis: a comprehensive review of metabolic engineering strategies inFrontiers in bioengineering and biotechnology · 2026Review
- Development of precise genome editing and multi-copy integration tools inSynthetic and systems biotechnology · 2025Article
- CRISPR/Cas9-based iterative multi-copy integration for improved metabolite yields inSynthetic and systems biotechnology · 2025Article
- Progress in the Metabolic Engineering ofBiodesign research · 2024Review
Corrections and comments
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Authors and funding
13 authors.
Funding
Abstract
The copy number of genes in chromosomes can be modified by chromosomal integration to construct efficient microbial cell factories but the resulting genetic systems are prone to failure or instability from triggering homologous recombination in repetitive DNA sequences. Finding the optimal copy number of each gene in a pathway is also time and labor intensive. To overcome these challenges, we applied a multiple nonrepetitive coding sequence calculator that generates sets of coding DNA sequence (CDS) variants. A machine learning method was developed to calculate the optimal copy number combination of genes in a pathway. We obtained an engineered Yarrowia lipolytica strain for eicosapentaenoic acid biosynthesis in 6 months, producing the highest titer of 27.5 g l
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Registered trials
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