Evidence map›Paper›PMID 38858133›Full record

ArticleFEBS letters2024

Creation of a novel CRISPR-generated allele to express HA epitope-tagged C1QL1 and improved methods for its detection at synapses.

Hiu W Cheung, Alexander D Schouw, Zeynep M Altunay, J Wesley Maddox, Lyndsay C Kresic, Brenna C McAllister, Keaven Caro, Shahnawaz Alam, Angie Huang, Robert S Pijewski and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FEBS letters, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Hiu W CheungDepartment of Neuroscience, University of Connecticut Health, Farmington, CT, USA.
Alexander D SchouwDepartment of Neuroscience, University of Connecticut Health, Farmington, CT, USA.
Zeynep M AltunayDepartment of Neuroscience, University of Connecticut Health, Farmington, CT, USA.ORCID https://orcid.org/0000-0002-7663-7417
J Wesley MaddoxDepartment of Neuroscience, University of Texas-Austin, TX, USA.ORCID https://orcid.org/0000-0002-1630-2746
Lyndsay C KresicDepartment of Neuroscience, University of Connecticut Health, Farmington, CT, USA.
Brenna C McAllisterDepartment of Neuroscience, University of Connecticut Health, Farmington, CT, USA.ORCID https://orcid.org/0000-0002-5567-7436
Keaven CaroDepartment of Neuroscience, University of Connecticut Health, Farmington, CT, USA.ORCID https://orcid.org/0000-0002-6179-8744
Shahnawaz AlamDepartment of Neuroscience, University of Connecticut Health, Farmington, CT, USA.ORCID https://orcid.org/0000-0002-7646-4392
Angie HuangDepartment of Neuroscience, University of Texas-Austin, TX, USA.ORCID https://orcid.org/0000-0001-8757-985X
Robert S PijewskiDepartment of Neuroscience, University of Connecticut Health, Farmington, CT, USA.ORCID https://orcid.org/0000-0002-5101-3587
Amy LeeDepartment of Neuroscience, University of Texas-Austin, TX, USA.
David C MartinelliDepartment of Neuroscience, University of Connecticut Health, Farmington, CT, USA.ORCID https://orcid.org/0000-0002-8318-3986

Funding

Calcium channels in retinal photoreceptorsR01EY026817 · NEI · UNIVERSITY OF TEXAS AT AUSTIN · PI LEE, AMY · 2017 to 2025
$4.4M
Determination of the subcellular localization of adhesion G protein-coupled receptor B3 (ADGRB3) and its locations of interaction with secreted C1Q-like ligandsR03DC019290 · NIDCD · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI MARTINELLI, DAVID CHRISTOPHER · 2020 to 2020
$164k
Common Fund R03DC019290Congressionally Directed Medical Research Programs W81XWH-21-1-0707/AConnecticut Institute for the Brain and Cognitive SciencesNEI NIH HHS R01 EY026817NIDCD NIH HHS R03 DC019290
6 · The paper itself

Abstract

C1QL1 is expressed in a subset of cells in the brain and likely has pleiotropic functions, including the regulation of neuron-to-neuron synapses. Research progress on C1QL proteins has been slowed by a dearth of available antibodies. Therefore, we created a novel knock-in mouse line in which an HA-tag is inserted into the endogenous C1ql1 locus. We examined the entire brain, identifying previously unappreciated nuclei expressing C1QL1, presumably in neurons. By total numbers, however, the large majority of C1QL1-expressing cells are of the oligodendrocyte lineage. Subcellular immunolocalization of synaptic cleft proteins is challenging, so we developed a new protocol to improve signal at synapses. Lastly, we compared various anti-HA antibodies to assist future investigations using this and likely other HA epitope-tagged alleles.

Indexed as

AllelesEpitopesSynapsesAnimalsBrainCRISPR-Cas SystemsGene Knock-In TechniquesMiceNerve Tissue ProteinsNeuronsEpitopesNerve Tissue ProteinsADGRB3BAI3CRISPR/Cas9Ctrp14oligodendrocytesynapse

Identifiers

PMID38858133
PMCPMC11479844

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.