Evidence map›Paper›PMID 38856895›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Purification of Potassium Ion Channels Using Styrene-Maleic Acid Copolymers.

Grigory Glukhov, Maria Karlova, Ekaterina Kravchuk, Anna Glukhova, Elizaveta Trifonova, Olga S Sokolova

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Grigory GlukhovFaculty of Biology, Shenzhen MSU-BIT University, Shenzhen, China.
Maria KarlovaFaculty of Biology, Moscow Lomonosov University, Moscow, Russia.
Ekaterina KravchukFaculty of Biology, Moscow Lomonosov University, Moscow, Russia.
Anna GlukhovaFaculty of Biology, Shenzhen MSU-BIT University, Shenzhen, China.
Elizaveta TrifonovaFaculty of Biology, Moscow Lomonosov University, Moscow, Russia.
Olga S SokolovaFaculty of Biology, Shenzhen MSU-BIT University, Shenzhen, China. sokolova@mail.bio.msu.ru.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Structural studies require the production of target proteins in large quantities and with a high degree of purity. For membrane proteins, the bottleneck in determining their structure is the extraction of the target protein from the cell membranes. A detergent that improperly mimics the hydrophobic environment of the protein of interest can also significantly alter its structure. Recently, using lipodiscs with styrene-maleic acid (SMA), copolymers became a promising strategy for the purification of membrane proteins. Here, we describe in detail the one-step affinity purification of potassium ion channels solubilized in SMA and sample preparation for future structural studies.

Indexed as

MaleatesPolystyrenesPotassium ChannelsChromatography, AffinityDetergentsHumansPolymersStyreneDetergentsMaleatesmaleic acidPolymersPolystyrenesPotassium ChannelsStyrenestyrene-maleic acid polymerAffinity purificationDLSElectron microscopyHuman Kv channelsSMALP

Identifiers

PMID38856895

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.