Evidence map›Paper›PMID 38855698›Full record

ArticleBiomedical optics express2024

Two-photon autofluorescence lifetime assay of rabbit photoreceptors and retinal pigment epithelium during light-dark visual cycles in rabbit retina.

Trung Duc Nguyen, Yuan-I Chen, Anh-Thu Nguyen, Siem Yonas, Manasa P Sripati, Yu-An Kuo, Soonwoo Hong, Mitchell Litvinov, Yujie He, Hsin-Chih Yeh and 1 more

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Article in Biomedical optics express, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

11 authors.

Trung Duc NguyenDepartment of Biomedical Engineering, University of Texas at Austin, Austin, TX, USA.
Yuan-I ChenDepartment of Biomedical Engineering, University of Texas at Austin, Austin, TX, USA.
Anh-Thu NguyenDepartment of Biomedical Engineering, University of Texas at Austin, Austin, TX, USA.ORCID https://orcid.org/0009-0005-7838-3317
Siem YonasDepartment of Biomedical Engineering, University of Texas at Austin, Austin, TX, USA.
Manasa P SripatiDepartment of Biomedical Engineering, University of Texas at Austin, Austin, TX, USA.
Yu-An KuoDepartment of Biomedical Engineering, University of Texas at Austin, Austin, TX, USA.
Soonwoo HongDepartment of Biomedical Engineering, University of Texas at Austin, Austin, TX, USA.ORCID https://orcid.org/0000-0002-7618-9052
Mitchell LitvinovDepartment of Biomedical Engineering, University of Texas at Austin, Austin, TX, USA.
Yujie HeDepartment of Biomedical Engineering, University of Texas at Austin, Austin, TX, USA.
Hsin-Chih YehDepartment of Biomedical Engineering, University of Texas at Austin, Austin, TX, USA.
H Grady RylanderDepartment of Biomedical Engineering, University of Texas at Austin, Austin, TX, USA.

Funding

Developing an ultrafast fluorescence lifetime imaging ophthalmoscopy system for retinal imagingR21EY033106 · NEI · UNIVERSITY OF TEXAS AT AUSTIN · PI YEH, HSIN-CHIH · 2021 to 2022
$399k
NEI NIH HHS R21 EY033106
6 · The paper itself

Abstract

Two-photon excited fluorescence (TPEF) is a powerful technique that enables the examination of intrinsic retinal fluorophores involved in cellular metabolism and the visual cycle. Although previous intensity-based TPEF studies in non-human primates have successfully imaged several classes of retinal cells and elucidated aspects of both rod and cone photoreceptor function, fluorescence lifetime imaging (FLIM) of the retinal cells under light-dark visual cycle has yet to be fully exploited. Here we demonstrate a FLIM assay of photoreceptors and retinal pigment epithelium (RPE) that reveals key insights into retinal physiology and adaptation. We found that photoreceptor fluorescence lifetimes increase and decrease in sync with light and dark exposure, respectively. This is likely due to changes in all-trans-retinol and all-trans-retinal levels in the outer segments, mediated by phototransduction and visual cycle activity. During light exposure, RPE fluorescence lifetime was observed to increase steadily over time, as a result of all-trans-retinol accumulation during the visual cycle and decreasing metabolism caused by the lack of normal perfusion of the sample. Our system can measure the fluorescence lifetime of intrinsic retinal fluorophores on a cellular scale, revealing differences in lifetime between retinal cell classes under different conditions of light and dark exposure.

Identifiers

PMID38855698
PMCPMC11161359

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.