Evidence map›Paper›PMID 38855659›Full record

ArticleBiomedical optics express2024

Piston-based specimen holder for rapid surgical and biopsy specimen imaging.

Chi Z Huang, Vincent D Ching-Roa, Connor M Heckman, William H Sipprell, Sherrif F Ibrahim, Bruce R Smoller, Michael G Giacomelli

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical optics express, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Chi Z HuangDepartment of Biomedical Engineering, University of Rochester, 207 Goergen Hall, Box 270168, Rochester, New York 14627, USA.ORCID https://orcid.org/0009-0002-5555-1232
Vincent D Ching-RoaDepartment of Biomedical Engineering, University of Rochester, 207 Goergen Hall, Box 270168, Rochester, New York 14627, USA.ORCID https://orcid.org/0000-0003-3836-534X
Connor M HeckmanDepartment of Biomedical Engineering, University of Rochester, 207 Goergen Hall, Box 270168, Rochester, New York 14627, USA.
William H SipprellRochester Dermatologic Surgery, PC, 7400 Pittsford Victor Rd Suite A, Victor, New York 14564, USA.
Sherrif F IbrahimRochester Dermatologic Surgery, PC, 7400 Pittsford Victor Rd Suite A, Victor, New York 14564, USA.
Bruce R SmollerDepartment of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, New York 14620, USA.
Michael G GiacomelliDepartment of Biomedical Engineering, University of Rochester, 207 Goergen Hall, Box 270168, Rochester, New York 14627, USA.ORCID https://orcid.org/0000-0002-2570-0770

Funding

Two photon fluorescence microscopy for dermatologic surgery and biopsyR37CA258376 · NCI · UNIVERSITY OF ROCHESTER · PI Michael Gene Giacomelli · 2021 to 2026
$2.0M
Two photon microscopy and optical clearing for volumetric imaging of melanoma surgical marginsR21EB032839 · NIBIB · UNIVERSITY OF ROCHESTER · PI GIACOMELLI, MICHAEL GENE · 2022 to 2023
$364k
NCI NIH HHS R37 CA258376NIBIB NIH HHS R21 EB032839
6 · The paper itself

Abstract

Advanced fluorescence imaging modalities such as confocal microscopy and two photon fluorescence microscopy can provide rapid, real-time histology images, but the mounting of fresh tissue specimens in standard orientations required for diagnosis without embedding and sectioning remains an unsolved problem. Here, we introduce a piston-based specimen holder designed for consistent, even pressure distribution. We improve upon previous designs by incorporating an air piston system with a flexible membrane and wick that extracts fluid during compression. We combine this with support fixtures to aid in the distribution of pressure, enabling imaging of specimens with small surface areas relative to their thickness, such as bisected shave skin biopsies in standard orientation without embedding or sectioning. We image both fresh biopsy specimens and diagnostic Mohs first stage specimens during clinical procedures, demonstrating improved visualization of the tissue surface in real time. Finally, we show that conventional cryosectioning can exaggerate the extent of margin positivity, which can be avoided using the piston-based holder.

Identifiers

PMID38855659
PMCPMC11161360

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.