Evidence map›Paper›PMID 38854050›Full record

ArticlebioRxiv : the preprint server for biology2025

An Unbiased Proteomic Platform for ATE1-based Arginylation Profiling.

Zongtao Lin, Yixuan Xie, Joanna Gongora, Xingyu Liu, Emily Zahn, Bibhuti Bhusana Palai, Daniel H Ramirez, Richard M Searfoss, Francisca N Vitorino, Rashmi Karki and 13 more

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PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

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2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

23 authors.

Zongtao LinDepartment of Biochemistry and Molecular Biophysics, Washington University in St. Louis, St. Louis, MO 63110.
Yixuan XieDepartment of Biochemistry and Molecular Biophysics, Washington University in St. Louis, St. Louis, MO 63110.
Joanna GongoraDepartment of Biochemistry and Molecular Biophysics, Washington University in St. Louis, St. Louis, MO 63110.
Xingyu LiuDepartment of Biochemistry and Molecular Biophysics, Washington University in St. Louis, St. Louis, MO 63110.
Emily ZahnDepartment of Biochemistry and Molecular Biophysics, Washington University in St. Louis, St. Louis, MO 63110.
Bibhuti Bhusana PalaiDepartment of Biochemistry and Molecular Biophysics, Washington University in St. Louis, St. Louis, MO 63110.
Daniel H RamirezDepartment of Biochemistry and Molecular Biophysics, Washington University in St. Louis, St. Louis, MO 63110.
Richard M SearfossDepartment of Biochemistry and Molecular Biophysics, Washington University in St. Louis, St. Louis, MO 63110.
Francisca N VitorinoDepartment of Biochemistry and Molecular Biophysics, Washington University in St. Louis, St. Louis, MO 63110.
Rashmi KarkiDepartment of Biochemistry and Molecular Biophysics, Washington University in St. Louis, St. Louis, MO 63110.
Geoffrey P DannDepartment of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA, 19104.
Chenfeng ZhaoMcKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO 63110.
Xian HanDepartment of Biochemistry and Molecular Biophysics, Washington University in St. Louis, St. Louis, MO 63110.
Brittany MacTaggartSchool of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104.
Xin LanDepartment of Biochemistry, Case Western Reserve University, Cleveland, OH 44106.
Dechen FuDepartment of Biochemistry, Case Western Reserve University, Cleveland, OH 44106.
Lina GreenbergDepartment of Biochemistry and Molecular Biophysics, Washington University in St. Louis, St. Louis, MO 63110.
Yi ZhangDepartment of Biochemistry, Case Western Reserve University, Cleveland, OH 44106.
Kory J LavineDepartment of Medicine, Washington University School of Medicine, St. Louis, MO, USA.
Michael J GreenbergDepartment of Biochemistry and Molecular Biophysics, Washington University in St. Louis, St. Louis, MO 63110.ORCID 0000-0003-1320-3547
Dongwen LvDepartment of Biochemistry and Structural Biology, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229.
Anna KashinaSchool of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104.ORCID 0000-0002-0243-6866
Benjamin A GarciaDepartment of Biochemistry and Molecular Biophysics, Washington University in St. Louis, St. Louis, MO 63110.

Funding

TISSUE CULTURE---COREP30CA054174 · NCI · UNIVERSITY OF TEXAS HLTH SCIENCE CENTER · PI Robin Jean Leach · 1991 to 2026
$59.1M
Research Education ComponentP30AG066444 · NIA · WASHINGTON UNIVERSITY · PI DAVID M. HOLTZMAN · 2020 to 2026
$28.7M
WU P&FP30DK020579 · NIDDK · WASHINGTON UNIVERSITY · PI Clay F. Semenkovich · 2013 to 2026
$27.1M
Regulation of cell migration by nucleotide coding sequence and arginylationR35GM122505 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI Anna S Kashina · 2017 to 2026
$6.2M
Specification and Function of Tissue Resident and Recruited Macrophages in Cardiac Remodeling and Heart FailureR35HL161185 · NHLBI · WASHINGTON UNIVERSITY · PI Kory J. Lavine · 2022 to 2026
$4.3M
Regulation of cardiac power output in health and diseaseR01HL141086 · NHLBI · WASHINGTON UNIVERSITY · PI GREENBERG, MICHAEL J · 2018 to 2022
$2.7M
Role of arginylation in prevention of alpha synuclein-driven neurodegenerationR01NS102435 · NINDS · UNIVERSITY OF PENNSYLVANIA · PI KASHINA, ANNA S · 2019 to 2023
$2.6M
Quantitative mass spectrometry for comprehending epigenetic mechanisms in a new underlying neurological developmental disorderR01HD106051 · NICHD · WASHINGTON UNIVERSITY · PI Benjamin A Garcia · 2022 to 2026
$2.6M
A Mass Spectrometry Approach to the Genetic and Epigenetic Mechanisms Controlling Neuronal IdentityR01NS111997 · NINDS · WEILL MEDICAL COLL OF CORNELL UNIV · PI DAHMANE, NADIA, GARCIA, BENJAMIN A · 2020 to 2024
$2.6M
Arginyl-tRNA beyond translation: mechanism and regulation of protein arginylationR35GM150678 · NIGMS · CASE WESTERN RESERVE UNIVERSITY · PI Yi Zhang · 2023 to 2026
$1.9M
Development and Application of Chemical Biology Approaches for Understanding Protein ArginylationR01HL177113 · NHLBI · WASHINGTON UNIVERSITY · PI Benjamin A Garcia, Zongtao Lin · 2025 to 2026
$1.2M
Hijacking Post-translational Arginylation for Targeted Protein DegradationR21CA292191 · NCI · WASHINGTON UNIVERSITY · PI LIN, ZONGTAO, LYU, DONGWEN · 2024 to 2024
$426k
NCI NIH HHS P30 CA054174NCI NIH HHS R21 CA286307NCI NIH HHS R21 CA292191NHLBI NIH HHS R01 HL141086NHLBI NIH HHS R01 HL177113NHLBI NIH HHS R35 HL161185NIA NIH HHS P30 AG066444NICHD NIH HHS R01 HD106051NIDDK NIH HHS P30 DK020579NIGMS NIH HHS R35 GM122505NIGMS NIH HHS R35 GM150678NINDS NIH HHS R01 NS102435NINDS NIH HHS R01 NS111997
6 · The paper itself

Abstract

Protein arginylation is an essential posttranslational modification (PTM) catalyzed by arginyl-tRNA-protein transferase 1 (ATE1) in mammalian systems. Arginylation features a post-translational conjugation of an arginyl to a protein, making it extremely challenging to differentiate from translational arginine residues with the same mass in a protein sequence. Here we present a general ATE1-based arginylation profiling platform for the unbiased discovery of arginylation substrates and their precise modification sites. This method integrates isotopic arginine labeling into an ATE1 assay utilizing biological lysates (

Indexed as

arginylationarginyltransferaseposttranslational modificationprotein profilingproteomics

Identifiers

PMID38854050
PMCPMC11160793

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.