Evidence map›Paper›PMID 38853519›Full record

ArticleDiabetes & metabolism journal2024

Single-Cell Landscape and a Macrophage Subset Enhancing Brown Adipocyte Function in Diabetes.

Junfei Gu, Jiajia Jin, Xiaoyu Ren, Xinjie Zhang, Jiaxuan Li, Xiaowei Wang, Shucui Zhang, Xianlun Yin, Qunye Zhang, Zhe Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Diabetes & metabolism journal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Junfei GuDepartment of Endocrinology & Geriatrics, Shandong Provincial Hospital, Shandong University, Jinan, China.
Jiajia JinNational Key Laboratory for Innovation and Transformation of Luobing Theory, Shandong University, Jinan, China.
Xiaoyu RenNational Key Laboratory for Innovation and Transformation of Luobing Theory, Shandong University, Jinan, China.
Xinjie ZhangDepartment of Biology, University College London, London, UK.
Jiaxuan LiDepartment of Endocrinology & Geriatrics, Shandong Provincial Hospital, Shandong University, Jinan, China.
Xiaowei WangNational Key Laboratory for Innovation and Transformation of Luobing Theory, Shandong University, Jinan, China.
Shucui ZhangNational Key Laboratory for Innovation and Transformation of Luobing Theory, Shandong University, Jinan, China.
Xianlun YinNational Key Laboratory for Innovation and Transformation of Luobing Theory, Shandong University, Jinan, China.
Qunye ZhangNational Key Laboratory for Innovation and Transformation of Luobing Theory, Shandong University, Jinan, China.
Zhe WangDepartment of Endocrinology & Geriatrics, Shandong Provincial Hospital, Shandong University, Jinan, China.

Funding

Clinical Medicine Technology Innovation Program of Jinan 202225044National Natural Science Foundation of China 82070820National Natural Science Foundation of China 82170495
6 · The paper itself

Abstract

backgruoundMetabolic dysregulation is a hallmark of type 2 diabetes mellitus (T2DM), in which the abnormalities in brown adipose tissue (BAT) play important roles. However, the cellular composition and function of BAT as well as its pathological significance in diabetes remain incompletely understood. Our objective is to delineate the single-cell landscape of BAT-derived stromal vascular fraction (SVF) and their characteristic alterations in T2DM rats.

methodsT2DM was induced in rats by intraperitoneal injection of low-dose streptozotocin and high-fat diet feeding. Single-cell mRNA sequencing was then performed on BAT samples and compared to normal rats to characterize changes in T2DM rats. Subsequently, the importance of key cell subsets in T2DM was elucidated using various functional studies.

resultsAlmost all cell types in the BAT-derived SVF of T2DM rats exhibited enhanced inflammatory responses, increased angiogenesis, and disordered glucose and lipid metabolism. The multidirectional differentiation potential of adipose tissue-derived stem cells was also reduced. Moreover, macrophages played a pivotal role in intercellular crosstalk of BAT-derived SVF. A novel Rarres2+macrophage subset promoted the differentiation and metabolic function of brown adipocytes via adipose-immune crosstalk.

conclusionBAT SVF exhibited strong heterogeneity in cellular composition and function and contributed to T2DM as a significant inflammation source, in which a novel macrophage subset was identified that can promote brown adipocyte function.

Indexed as

Adipocytes, BrownAdipose Tissue, BrownDiabetes Mellitus, ExperimentalDiabetes Mellitus, Type 2MacrophagesSingle-Cell AnalysisAnimalsCell DifferentiationDiet, High-FatMaleRatsRats, Sprague-DawleyAdipocytes, brownAdipose tissue, brownDiabetes mellitus, type 2MacrophagesSingle-cell gene expression analysis

Identifiers

PMID38853519
PMCPMC11449828

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.