ArticleCurrent microbiology2024
Assessment of Fungal Lytic Enzymatic Extracts Produced Under Submerged Fermentation as Enhancers of Entomopathogens' Biological Activity.
Article in Current microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Optimization of Culture Media for Trichoderma Strains as a Sustainable Approach for Agriculture.Current microbiology · 2026Article
- Sustainable production of insecticidal and acaricidal metabolites by endophytic fungi using solid-state fermentation.Scientific reports · 2026Article
- Towards a Novel Biocontrol Strategy: High Performance of Optimised Cell Wall-Degrading Enzymes Secreted by Escovopsis primorosea LBM 277.Environmental microbiology reports · 2026Article
- Biomass and Extracts of Metarhizium robertsii MT008 as Potential Biopesticides for Controlling the Fruit Fly Anastrepha obliqua.Neotropical entomology · 2025Article
- Morphological, Physiological, Biochemical, and Molecular Characterization of Fungal Species Associated with Papaya Rot in Cameroon.Journal of fungi (Basel, Switzerland) · 2025Article
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Authors and funding
5 authors.
Funding
Abstract
The application of enzymes in agricultural fields has been little explored. One potential application of fungal lytic enzymes (chitinases, lipases, and proteases) is as an additive to current biopesticides to increase their efficacy and reduce the time of mortality. For this, a screening of lytic overproducer fungi under submerged fermentation with a chemical-defined medium was performed. Then, the enzymatic crude extract (ECE) was concentrated and partially characterized. This characterization consisted of measuring the enzymatic activity (lipase, protease and, chitinase) and determining the enzyme stability after storage at temperatures of - 80, - 20 and, 4 °C. And lastly, the application of these concentrated enzymatic crude extracts (C-ECE) as an enhancer of spores-based fungal biopesticide was proven. Beauveria were not as good producers of lytic enzymes as the strains from Trichoderma and Metarhizium. The isolate M. robertsii Mt015 was selected for the co-production of chitinases and proteases; and the isolate T. harzianum Th180 for co-production of chitinases, lipases, and proteases. The C-ECE of Mt015 had a protease activity of 18.6 ± 1.1 U ml
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