Factors affecting protein recovery during Hsp40 affinity profiling.

Maureen R Montoya, Guy M Quanrud, Liangyong Mei, José L Moñtano, Caleb Hong, Joseph C Genereux

Abstract read

In one paragraph

Article in Analytical and bioanalytical chemistry, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Maureen R MontoyaDepartment of Chemistry, University of California, 501 Big Springs Rd, Riverside, CA, 92521, USA.

Guy M QuanrudDepartment of Chemistry, University of California, 501 Big Springs Rd, Riverside, CA, 92521, USA.

Liangyong MeiDepartment of Chemistry, University of North Florida, Jacksonville, FL, USA.

José L MoñtanoDepartment of Chemistry, University of California, 501 Big Springs Rd, Riverside, CA, 92521, USA.

Caleb HongDepartment of Chemistry, University of California, 501 Big Springs Rd, Riverside, CA, 92521, USA.

Joseph C GenereuxDepartment of Chemistry, University of California, 501 Big Springs Rd, Riverside, CA, 92521, USA. josephg@ucr.edu.ORCID http://orcid.org/0000-0002-5093-7710

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The identification and quantification of misfolded proteins from complex mixtures is important for biological characterization and disease diagnosis, but remains a major bioanalytical challenge. We have developed Hsp40 Affinity Profiling as a bioanalytical approach to profile protein stability in response to cellular stress. In this assay, we ectopically introduce the Hsp40

Indexed as

HSP40 Heat-Shock ProteinsHEK293 CellsHSP70 Heat-Shock ProteinsHumansProtein BindingProtein FoldingProtein StabilityProteomicsDNAJB1 protein, humanHSP40 Heat-Shock ProteinsHSP70 Heat-Shock ProteinsAP-MSDNAJB1DNAJB8Hsp40Misfolded proteinsProteomics

Identifiers

PMID38850318

DOI10.1016/j.jmb.2021.167197

PMCPMC11271386

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.