Evidence map›Paper›PMID 38849609›Full record

ArticleFunctional & integrative genomics2024

Multi-omics analysis reveals mechanism of Schisandra chinensis lignans and acteoside on EMT in hepatoma cells via ERK1/2 pathway.

Jingjing Jiang, Ru Cheng, Aoqi Song, Yuefen Lou, Guorong Fan

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Article in Functional & integrative genomics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

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5citing papers in PubMed
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3 · Its place in the literature

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5 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Jingjing JiangDepartment of Pharmacy, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai, 200434, China.
Ru ChengDepartment of Pharmacy, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai, 200434, China.
Aoqi SongDepartment of Pharmacy, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai, 200434, China.
Yuefen LouDepartment of Pharmacy, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai, 200434, China. louyuefen@sina.cn.
Guorong FanDepartment of Clinical Pharmacy, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200080, China. fanguorong@sjtu.edu.cn.

Funding

Project of Discipline Promotion Program of Shanghai Fourth People's Hospital SY-XKZT-2020-1014Research Project of Shanghai Municipal Health Commission 20194Y0202Research project on traditional Chinese medicine of Hongkou District Health Commission HKQ-ZYY-2021-33
6 · The paper itself

Abstract

backgroundHepatocellular carcinoma (HCC), a globally common cancer, often presents late and shows high resistance to chemotherapy, resulting in suboptimal treatment efficacy. Components from traditional Chinese medicines have been recognized for their anti-cancer properties.

objectiveExploring the mechanism of Schisandra chinensis lignans and acteoside in suppressing Epithelial-Mesenchymal Transition (EMT) in hepatoma cells through the Extracellular signal-Regulated Kinases (ERK)1/2 pathway and identifying biomarkers, molecular subtypes, and targets via multi-omics for precision oncology.

methodsProliferation was assessed using cell counting kit-8 (CCK-8) assays, with scratch and transwell assays for evaluating invasion and migration. Flow cytometry quantified apoptosis rates. Expression levels of CCL20, p-ERK1/2, c-Myc, Vimentin, and E-cadherin/N-cadherin were analyzed by real-time PCR and Western blot. Tumor volume was calculated with a specific formula, and growth.

resultsThe Schisandra chinensis lignans and acteoside combination decreased CCL20 expression, inhibited hepatoma proliferation and migration, and enhanced apoptosis in a dose- and time-dependent manner. Molecular analysis revealed increased E-cadherin and decreased N-cadherin, p-ERK1/2, c-Myc, and Vimentin expression, indicating ERK1/2 pathway modulation. In vivo, treated nude mice showed significantly reduced tumor growth and volume.

conclusionSchisandra chinensis lignans and acteoside potentially counteract CCL20-induced EMT, invasion, and migration in hepatocellular carcinoma cells via the ERK1/2 pathway, enhancing apoptosis. Multi-omics analysis further aids in pinpointing novel biomarkers for precision cancer therapy.

Indexed as

ApoptosisCarcinoma, HepatocellularCell ProliferationEpithelial-Mesenchymal TransitionGlucosidesLignansLiver NeoplasmsMAP Kinase Signaling SystemPhenolsSchisandraAnimalsCell Line, TumorCell MovementChemokine CCL20Hep G2 CellsHumansacteosideChemokine CCL20GlucosidesLignansPhenolsPolyphenolsActeosideEpithelial-mesenchymal transitionExtracellular regulated kinases 1/2 pathwayLiver cancerSchisandra chinensis lignans

Identifiers

PMID38849609

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