ArticleBiology of reproduction2024
Male infertility and perfluoroalkyl and poly-fluoroalkyl substances: evidence for alterations in phosphorylation of proteins and fertility-related functional attributes in bull spermatozoa†.
Article in Biology of reproduction, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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Who cites it
7 citing papers in PubMed.
- Impact of Cadmium Toxicity on Testicular Function: Risk of Male Infertility.Life (Basel, Switzerland) · 2026Review
- Environmental and external stressors of postejaculatory sperm function.The Journal of reproduction and development · 2026Review
- Review
- Unveiling the reproductive risks of PFOS: testicular proteome disruption and hormonal imbalance in male rats†.Biology of reproduction · 2025Article
- Molecular and Computational Studies Reveal That Per- and Polyfluoroalkyl Substances Can Impair Protamine-DNA Interaction, Potentially Inducing DNA Damage.Biomolecules · 2025Article
- Article
- PFAS Exposure and Male Reproductive Health: Implications for Sperm Epigenetics.Seminars in reproductive medicine · 2024Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
backgroundPerfluoroalkyl and poly-fluoroalkyl substances (PFAS) are pervasive environmental pollutants and potential threats to reproductive health. Epidemiological studies have established an association between PFAS and male infertility, but the underlying mechanisms are unclear.
objectivesInvestigate the effect of perfluorooctane sulfonic acid (PFOS), the most prevalent and representative PFAS, on bull sperm protein phosphorylation and function.
methodsWe exposed bull sperm to PFOS at 10 (average population exposure) and 100 μM (high-exposure scenario), and analyzed global proteomic and phosphoproteomic analysis by TMT labeling and Nano LC-MS/MS. We also measured sperm fertility functions by flow cytometry.
resultsPFOS at 10-μM altered sperm proteins linked to spermatogenesis and chromatin condensation, while at 100 μM, PFOS affected proteins associated with motility and fertility. We detected 299 phosphopeptides from 116 proteins, with 45 exhibiting differential expression between control and PFOS groups. PFOS dysregulated phosphorylation of key proteins (ACRBP, PRKAR2A, RAB2B, SPAG8, TUBB4B, ZPBP, and C2CD6) involved in sperm capacitation, acrosome reaction, sperm-egg interaction, and fertilization. PFOS also affected phosphorylation of other proteins (AQP7, HSBP9, IL4I1, PRKAR1A, and CCT8L2) related to sperm stress resistance and cryotolerance. Notably, four proteins (PRM1, ACRBP, TSSK1B, and CFAP45) exhibited differential regulation at both proteomic and phosphoproteomic levels. Flow cytometric analysis confirmed that PFOS increased protein phosphorylation in sperm and also decreased sperm motility, viability, calcium, and mitochondrial membrane potential and increased mitochondrial ROS in a dose-dependent manner.
conclusionsThis study demonstrates that PFOS exposure negatively affects phosphorylation of proteins vital for bull sperm function and fertilization.
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