Evidence map›Paper›PMID 38844639›Full record

ArticleBiochemical genetics2025

miR-146b-5p Regulates the Enteric Nervous System Development in Hirschsprung Disease via Targeting RET Pathway.

Bobin Zhang, Jian Yang, Aohua Song, Wei Feng, Zhenhua Guo

Abstract read
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In one paragraph

Article in Biochemical genetics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Bobin Zhang *Ministry of Education Key Laboratory of Child Development, Chongqing Key Laboratory of Structural Birth Defect and Reconstruction, National Clinical Research Center for Child Health and Disorders, Department of Neonatal Surgery, Children's Hospital of Chongqing Medical University, 20, Jinyu Road, Yubei District, Chongqing, 400025, People's Republic of China.
Jian Yang *Department of General Surgery, Yongchuan Maternal and Child Health Hospital, Chongqing, People's Republic of China.
Aohua SongMinistry of Education Key Laboratory of Child Development, Chongqing Key Laboratory of Structural Birth Defect and Reconstruction, National Clinical Research Center for Child Health and Disorders, Department of Neonatal Surgery, Children's Hospital of Chongqing Medical University, 20, Jinyu Road, Yubei District, Chongqing, 400025, People's Republic of China.
Wei FengMinistry of Education Key Laboratory of Child Development, Chongqing Key Laboratory of Structural Birth Defect and Reconstruction, National Clinical Research Center for Child Health and Disorders, Department of Neonatal Surgery, Children's Hospital of Chongqing Medical University, 20, Jinyu Road, Yubei District, Chongqing, 400025, People's Republic of China.
Zhenhua GuoMinistry of Education Key Laboratory of Child Development, Chongqing Key Laboratory of Structural Birth Defect and Reconstruction, National Clinical Research Center for Child Health and Disorders, Department of Neonatal Surgery, Children's Hospital of Chongqing Medical University, 20, Jinyu Road, Yubei District, Chongqing, 400025, People's Republic of China. zhguo@cqmu.edu.cn.

Funding

Natural Science Foundation Project of Chongqing, Chongqing Science and Technology Commission CSTC2020jcyj-msxmX0249
6 · The paper itself

Abstract

The objective of the study is to investigate how miR-146b-5p might contribute to the etiology of HSCR. The study investigated the expression levels of miRNA, mRNA, and proteins in colon tissues obtained from the HSCR and control groups. The role of miR-146b-5p in cell proliferation and migration was studied in vitro. The interaction between miR-146b-5p and RET was validated through a dual-luciferase reporter experiment. To assess the impact of miR-146b-5p on the development of the enteric nervous system, zebrafish embryos were micro-injected with either miR-146b-5p mimics or negative control, followed by subsequent evaluation. Compared to the control group, miR-146b-5p expression levels in the spastic region of HSCR were significantly increased. In vitro, miR-146b-5p prevented cell migration and proliferation by targeting RET pathway. In zebrafish, miR-146b-5p negatively regulates the migration of neural crest cells through a reduction in RET expression. Overexpression of miR-146b-5p hinders the development of mature neurons by decreasing RET expression. Additionally, the aberrant phenotypes induced by miR-146b-5p were partially ameliorated when RET mRNA was co-injected. By targeting RET in HSCR patients, aberrant expression of miR-146b-5p may play a unique role in the etiology of the disease and be involved in enteric nervous system development.

Indexed as

Enteric Nervous SystemHirschsprung DiseaseMicroRNAsProto-Oncogene Proteins c-retAnimalsCell MovementCell ProliferationHumansSignal TransductionZebrafishMicroRNAsMIRN146 microRNA, humanProto-Oncogene Proteins c-retRET protein, humanHirschsprung diseaseMigrationmiR-146b-5pProliferationRET

Identifiers

PMID38844639

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.