Evidence map›Paper›PMID 38844552›Full record

ReviewNature protocols2024

Scanorama: integrating large and diverse single-cell transcriptomic datasets.

Brian L Hie, Soochi Kim, Thomas A Rando, Bryan Bryson, Bonnie Berger

Abstract readReview
In one paragraph

Review in Nature protocols, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed.

  1. Article
  2. LIGER2: Scalable Single-Cell Integration with On-Disk Datasets.bioRxiv : the preprint server for biology · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Brian L Hie *Department of Chemical Engineering, Stanford University School, Stanford, CA, USA. brianhie@stanford.edu.ORCID 0000-0003-3224-8142
Soochi Kim *Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA, USA.
Thomas A RandoDepartment of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA, USA.ORCID 0000-0001-5843-8564
Bryan BrysonDepartment of Biological Engineering, MIT, Cambridge, MA, USA. bryand@mit.edu.
Bonnie BergerComputer Science and Artificial Intelligence Laboratory, MIT, Cambridge, MA, USA. bab@mit.edu.ORCID 0000-0002-2724-7228

Funding

Aging and Stem Cell ResilienceR01AG068667 · NIA · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI RANDO, THOMAS A. · 2021 to 2025
$2.8M
Manifold representations and active learning for 21 st century biologyR35GM141861 · NIGMS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BERGER, BONNIE · 2021 to 2025
$1.9M
NIA NIH HHS R01 AG068667NIGMS NIH HHS R35 GM141861
6 · The paper itself

Abstract

Merging diverse single-cell RNA sequencing (scRNA-seq) data from numerous experiments, laboratories and technologies can uncover important biological insights. Nonetheless, integrating scRNA-seq data encounters special challenges when the datasets are composed of diverse cell type compositions. Scanorama offers a robust solution for improving the quality and interpretation of heterogeneous scRNA-seq data by effectively merging information from diverse sources. Scanorama is designed to address the technical variation introduced by differences in sample preparation, sequencing depth and experimental batches that can confound the analysis of multiple scRNA-seq datasets. Here we provide a detailed protocol for using Scanorama within a Scanpy-based single-cell analysis workflow coupled with Google Colaboratory, a cloud-based free Jupyter notebook environment service. The protocol involves Scanorama integration, a process that typically spans 0.5-3 h. Scanorama integration requires a basic understanding of cellular biology, transcriptomic technologies and bioinformatics. Our protocol and new Scanorama-Colaboratory resource should make scRNA-seq integration more widely accessible to researchers.

Indexed as

Single-Cell AnalysisTranscriptomeComputational BiologyGene Expression ProfilingHumansRNA-SeqSequence Analysis, RNASoftware

Identifiers

PMID38844552
PMCPMC11361826

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.