Evidence map›Paper›PMID 38844464›Full record

ArticleNature communications2024

m6a methylation orchestrates IMP1 regulation of microtubules during human neuronal differentiation.

Pierre Klein, Marija Petrić Howe, Jasmine Harley, Harry Crook, Sofia Esteban Serna, Theodoros I Roumeliotis, Jyoti S Choudhary, Anob M Chakrabarti, Raphaëlle Luisier, Rickie Patani and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Genetic regulators of neuronal survival across metabolic environments.bioRxiv : the preprint server for biology · 2025
    Article
  6. Review
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Pierre KleinDivision of Biosciences, Institute of Structural and Molecular Biology, University College London, Darwin Building, Gower Street, London, WC1E 6XA, UK.ORCID http://orcid.org/0000-0002-9209-2939
Marija Petrić HoweHuman Stem Cells and Neurodegeneration Laboratory, The Francis Crick Institute, 1 Midland Road, London, NW1 1AT, UK.
Jasmine HarleyHuman Stem Cells and Neurodegeneration Laboratory, The Francis Crick Institute, 1 Midland Road, London, NW1 1AT, UK.
Harry CrookHuman Stem Cells and Neurodegeneration Laboratory, The Francis Crick Institute, 1 Midland Road, London, NW1 1AT, UK.
Sofia Esteban SernaDivision of Biosciences, Institute of Structural and Molecular Biology, University College London, Darwin Building, Gower Street, London, WC1E 6XA, UK.ORCID http://orcid.org/0000-0002-8770-3264
Theodoros I RoumeliotisFunctional Proteomics team, The Institute of Cancer Research, 237 Fulham Road, London, SW3 6JB, UK.ORCID http://orcid.org/0000-0002-3354-5643
Jyoti S ChoudharyFunctional Proteomics team, The Institute of Cancer Research, 237 Fulham Road, London, SW3 6JB, UK.ORCID http://orcid.org/0000-0003-0881-5477
Anob M ChakrabartiRNA Networks Laboratory, The Francis Crick Institute, 1 Midland Road, London, NW1 1AT, UK.ORCID http://orcid.org/0000-0002-6841-5718
Raphaëlle LuisierIdiap Research Institute, Martigny, 1920, Switzerland.
Rickie PataniHuman Stem Cells and Neurodegeneration Laboratory, The Francis Crick Institute, 1 Midland Road, London, NW1 1AT, UK. rickie.patani@ucl.ac.uk.ORCID http://orcid.org/0000-0002-3825-7675
Andres RamosDivision of Biosciences, Institute of Structural and Molecular Biology, University College London, Darwin Building, Gower Street, London, WC1E 6XA, UK. a.ramos@ucl.ac.uk.ORCID http://orcid.org/0000-0002-9295-8042

Funding

Medical Research Council MR/S000305/1RCUK | Biotechnology and Biological Sciences Research Council (BBSRC) S014438RCUK | Medical Research Council (MRC) S000305RCUK | Medical Research Council (MRC) S006591Wellcome Trust
6 · The paper itself

Abstract

Neuronal differentiation requires building a complex intracellular architecture, and therefore the coordinated regulation of defined sets of genes. RNA-binding proteins (RBPs) play a key role in this regulation. However, while their action on individual mRNAs has been explored in depth, the mechanisms used to coordinate gene expression programs shaping neuronal morphology are poorly understood. To address this, we studied how the paradigmatic RBP IMP1 (IGF2BP1), an essential developmental factor, selects and regulates its RNA targets during the human neuronal differentiation. We perform a combination of system-wide and molecular analyses, revealing that IMP1 developmentally transitions to and directly regulates the expression of mRNAs encoding essential regulators of the microtubule network, a key component of neuronal morphology. Furthermore, we show that m6A methylation drives the selection of specific IMP1 mRNA targets and their protein expression during the developmental transition from neural precursors to neurons, providing a molecular principle for the onset of target selectivity.

Indexed as

Cell DifferentiationMicrotubulesNeuronsRNA-Binding ProteinsRNA, MessengerAdenosineGene Expression Regulation, DevelopmentalHumansMethylationNeurogenesisAdenosineIGF2BP1 protein, humanRNA-Binding ProteinsRNA, Messenger

Identifiers

PMID38844464
PMCPMC11156911

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.