Evidence map›Paper›PMID 38842297›Full record

ArticleJournal of veterinary internal medicine

Blood-brain barrier dysfunction and decreased transcription of tight junction proteins in epileptic dogs.

Erez Hanael, Shelly Baruch, Rotem Kalev Altman, Orit Chai, Kira Rapoport, Dana Peery, Alon Friedman, Merav H Shamir

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Article in Journal of veterinary internal medicine. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Erez HanaelKoret School of Veterinary Medicine, Neurology and Neurosurgery, The Hebrew University of Jerusalem, Rehovot, Israel.ORCID https://orcid.org/0000-0003-1284-895X
Shelly BaruchKoret School of Veterinary Medicine, The Robert H. Smith Faculty of Agriculture, Food and Environment, Neurology and Neurosurgery, The Hebrew University of Jerusalem, Rehovot, Israel.
Rotem Kalev AltmanKoret School of Veterinary Medicine, The Hebrew University of Jerusalem, Rehovot, Israel.
Orit ChaiKoret School of Veterinary Medicine, The Hebrew University of Jerusalem, Rehovot, Israel.
Kira RapoportKoret School of Veterinary Medicine, The Robert H. Smith Faculty of Agriculture, Food and Environment, Neurology and Neurosurgery, The Hebrew University of Jerusalem, Rehovot, Israel.
Dana PeeryKoret School of Veterinary Medicine, The Hebrew University of Jerusalem, Rehovot, Israel.
Alon FriedmanBen-Gurion University of the Negev, Beer-Sheva, Israel.
Merav H ShamirKoret School of Veterinary Medicine, The Robert H. Smith Faculty of Agriculture, Food and Environment, Neurology and Neurosurgery, The Hebrew University of Jerusalem, Rehovot, Israel.ORCID https://orcid.org/0000-0002-4698-5373

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundEpilepsy in dogs and humans is associated with blood-brain barrier (BBB) dysfunction (BBBD), which may involve dysfunction of tight junction (TJ) proteins, matrix metalloproteases, and astrocytes. Imaging techniques to assess BBB integrity, to identify potential treatment strategies, have not yet been evaluated in veterinary medicine. HYPOTHESIS: Some dogs with idiopathic epilepsy (IE) will exhibit BBBD. Identifying BBBD may improve antiepileptic treatment in the future. ANIMALS: Twenty-seven dogs with IE and 10 healthy controls.

methodsRetrospective, prospective cohort study. Blood-brain barrier permeability (BBBP) scores were calculated for the whole brain and piriform lobe of all dogs by using dynamic contrast enhancement (DCE) magnetic resonance imaging (MRI) and subtraction enhancement analysis (SEA). Matrix metalloproteinase-9 (MMP9) activity in serum and cerebrospinal fluid (CSF) was measured and its expression in the piriform lobe was examined using immunofluorescent staining. Gene expression of TJ proteins and astrocytic transporters was analyzed in the piriform lobe.

resultsThe DCE-MRI analysis of the piriform lobe identified higher BBBP score in the IE group when compared with controls (34.5% vs 26.5%; P = .02). Activity and expression of MMP9 were increased in the serum, CSF, and piriform lobe of IE dogs as compared with controls. Gene expression of Kir4.1 and claudin-5 in the piriform lobe of IE dogs was significantly lower than in control dogs. CONCLUSIONS AND CLINICAL IMPORTANCE: Our findings demonstrate BBBD in dogs with IE and were supported by increased MMP9 activity and downregulation of astrocytic potassium channels and some TJ proteins. Blood brain barrier dysfunction may be a novel antiepileptic therapy target.

Indexed as

Blood-Brain BarrierDog DiseasesEpilepsyMagnetic Resonance ImagingMatrix Metalloproteinase 9Tight Junction ProteinsAnimalsCase-Control StudiesCohort StudiesDogsFemaleMaleProspective StudiesRetrospective StudiesMatrix Metalloproteinase 9Tight Junction Proteinsblood‐brain barrierclaudin 5CNS disordersepilepsyKir 4.1MMP9neurologytight junction

Identifiers

PMID38842297
PMCPMC11256172

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.