ArticleVeterinary pathology2024
Effects of fixation and demineralization on histomorphology and DNA amplification of canine bone marrow.
Article in Veterinary pathology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Effects of fixation and demineralization on immunohistochemical assessment of canine bone marrow.Veterinary pathology · 2026Article
- A comparison of laser, electrosurgical, and mechanical transcatheter traversal of calcified aortic valve leaflets.JTCVS structural and endovascular · 2025Article
- Case Report: Pitfalls in bone marrow evaluation: importance of adequate bone marrow sampling.Frontiers in oncology · 2025Article
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Authors and funding
7 authors.
Funding
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Abstract
Fixation and demineralization protocols for bone marrow (BM) across diagnostic laboratories are not standardized. How different protocols affect histomorphology and DNA amplification is incompletely understood. In this study, 2 fixatives and 3 demineralization methods were tested on canine BM samples. Twenty replicate sternal samples obtained within 24 hours of death were fixed overnight in either acetic acid-zinc-formalin (AZF) or 10% neutral-buffered formalin (NBF) and demineralized with formic acid for 12 hours. Another 53 samples were fixed in AZF and demineralized with hydrochloric acid for 1-hour, formic acid for 12 hours, or ethylenediamine tetraacetic acid (EDTA) for 24 hours. Histologic sections were scored by 4 raters as of insufficient, marginal, good, or excellent quality. In addition, DNA samples extracted from sections treated with the different fixation and demineralization methods were amplified with 3 sets of primers to conserved regions of
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