Evidence map›Paper›PMID 38841912›Full record

ArticleBiology open2024

Re-assessment of the subcellular localization of Bazooka/Par-3 in Drosophila: no evidence for localization to the nucleus and the neuromuscular junction.

Soya Kim, Jaffer Shahab, Elisabeth Vogelsang, Andreas Wodarz

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Article in Biology open, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

4 authors.

Soya KimMolecular Cell Biology, Center for Anatomy, University of Cologne and University Hospital Cologne, Weyertal 115c, 50931 Köln, Germany.
Jaffer ShahabStem Cell Biology, Institute for Anatomy and Cell Biology, Georg-August-University Göttingen, Justus-von-Liebig-Weg 11, 37077 Göttingen, Germany.
Elisabeth VogelsangMolecular Cell Biology, Center for Anatomy, University of Cologne and University Hospital Cologne, Weyertal 115c, 50931 Köln, Germany.
Andreas WodarzMolecular Cell Biology, Center for Anatomy, University of Cologne and University Hospital Cologne, Weyertal 115c, 50931 Köln, Germany.ORCID 0000-0002-0007-6289

Funding

Deutsche Forschungsgemeinschaft Cluster of Excellence 171 Nanoscale Microscopy and Molecular Physiology of the BrainDeutsche Forschungsgemeinschaft Cluster of Excellence 171 "Nanoscale Microscopy and Molecular Physiology of the Brain"University of Cologne: Universität zu Köln
6 · The paper itself

Abstract

Bazooka/Par-3 (Baz) is an evolutionarily conserved scaffold protein that functions as a master regulator for the establishment and maintenance of cell polarity in many different cell types. In the vast majority of published research papers Baz has been reported to localize at the cell cortex and at intercellular junctions. However, there have also been several reports showing localization and function of Baz at additional subcellular sites, in particular the nuclear envelope and the neuromuscular junction. In this study we have re-assessed the localization of Baz to these subcellular sites in a systematic manner. We used antibodies raised in different host animals against different epitopes of Baz for confocal imaging of Drosophila tissues. We tested the specificity of these antisera by mosaic analysis with null mutant baz alleles and tissue-specific RNAi against baz. In addition, we used a GFP-tagged gene trap line for Baz and a bacterial artificial chromosome (BAC) expressing GFP-tagged Baz under control of its endogenous promoter in a baz mutant background to compare the subcellular localization of the GFP-Baz fusion proteins to the staining with anti-Baz antisera. Together, these experiments did not provide evidence for specific localization of Baz to the nucleus or the neuromuscular junction.

Indexed as

Cell NucleusDrosophila melanogasterDrosophila ProteinsNeuromuscular JunctionAnimalsIntracellular Signaling Peptides and ProteinsProtein Transportbaz protein, DrosophilaDrosophila ProteinsIntracellular Signaling Peptides and ProteinsBazooka/Par-3Neuromuscular junctionNuclear localizationSubcellular localization

Identifiers

PMID38841912
PMCPMC11225583

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.