Evidence map›Paper›PMID 38841422›Full record

ArticleNeurophotonics2024

Two-dimensional electro-optical multiphoton microscopy.

Deano M Farinella, Samuel Stanek, Harishankar Jayakumar, Zachary L Newman, Jacob Gable, James Leger, Aaron Kerlin

Abstract read
In one paragraph

Article in Neurophotonics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Deano M FarinellaUniversity of Minnesota, Department of Neuroscience, Minneapolis, Minnesota, United States.ORCID https://orcid.org/0000-0003-0312-0801
Samuel StanekUniversity of Minnesota, Department of Electrical and Computer Engineering, Minneapolis, Minnesota, United States.
Harishankar JayakumarUniversity of Minnesota, Department of Neuroscience, Minneapolis, Minnesota, United States.
Zachary L NewmanUniversity of Minnesota, Department of Neuroscience, Minneapolis, Minnesota, United States.ORCID https://orcid.org/0000-0002-4336-6585
Jacob GableUniversity of Minnesota, Department of Neuroscience, Minneapolis, Minnesota, United States.
James LegerUniversity of Minnesota, Department of Electrical and Computer Engineering, Minneapolis, Minnesota, United States.ORCID https://orcid.org/0000-0001-9319-7196
Aaron KerlinUniversity of Minnesota, Department of Neuroscience, Minneapolis, Minnesota, United States.

Funding

Imaging at the speed of spikes: An electro-optical multiphoton microscopeRF1NS128658 · NINDS · UNIVERSITY OF MINNESOTA · PI KERLIN, AARON MICHAEL · 2022 to 2022
$2.0M
NINDS NIH HHS RF1 NS128658
6 · The paper itself

Abstract

Significance: The development of genetically encoded fluorescent indicators of neural activity with millisecond dynamics has generated demand for ever faster two-photon (2P) imaging systems, but acoustic and mechanical beam scanning technologies are approaching fundamental limits. We demonstrate that potassium tantalate niobate (KTN) electro-optical deflectors (EODs), which are not subject to the same fundamental limits, are capable of ultrafast two-dimensional (2D) 2P imaging Aim: To determine if KTN-EODs are suitable for 2P imaging, compatible with 2D scanning, and capable of ultrafast Approach: The performance of a commercially available KTN-EOD was characterized across a range of drive frequencies and laser parameters relevant to Results: Optimal KTN-EOD deflection of laser light with a central wavelength of 960 nm was obtained up to the highest average powers and pulse intensities tested (power: 350 mW; pulse duration: 118 fs). Up to 32 resolvable spots per line at a 560 kHz line scan rate could be obtained with single-axis deflection. The complete dual-axis EO 2P microscope was capable of imaging a Conclusions: We demonstrate the suitability of KTN-EODs for ultrafast 2P cellular imaging

Indexed as

electro-optical deflectorin vivo imagingmultiphoton microscopyvoltage imaging

Identifiers

PMID38841422
PMCPMC11151658

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.