Evidence map›Paper›PMID 38840117›Full record

ArticleCancer cell international2024

Assessing the impact of extracellular matrix fiber orientation on breast cancer cellular metabolism.

Madison R Pickett, Yuan-I Chen, Mohini Kamra, Sachin Kumar, Nikhith Kalkunte, Gabriella P Sugerman, Kelsey Varodom, Manuel K Rausch, Janet Zoldan, Hsin-Chin Yeh and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer cell international, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Collagen remodeling in breast cancer progression: from molecular mechanisms to diagnostic and therapeutic opportunities.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2026
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Madison R PickettDepartment of Biomedical Engineering, The University of Texas at Austin, 107 W Dean Keeton Street Stop C0800, Austin, TX, 78712, USA. mrpickett@uexas.edu.
Yuan-I ChenDepartment of Biomedical Engineering, The University of Texas at Austin, 107 W Dean Keeton Street Stop C0800, Austin, TX, 78712, USA.
Mohini KamraDepartment of Biomedical Engineering, The University of Texas at Austin, 107 W Dean Keeton Street Stop C0800, Austin, TX, 78712, USA.
Sachin KumarDepartment of Biomedical Engineering, The University of Texas at Austin, 107 W Dean Keeton Street Stop C0800, Austin, TX, 78712, USA.
Nikhith KalkunteDepartment of Biomedical Engineering, The University of Texas at Austin, 107 W Dean Keeton Street Stop C0800, Austin, TX, 78712, USA.
Gabriella P SugermanDepartment of Biomedical Engineering, The University of Texas at Austin, 107 W Dean Keeton Street Stop C0800, Austin, TX, 78712, USA.
Kelsey VarodomDepartment of Biomedical Engineering, The University of Texas at Austin, 107 W Dean Keeton Street Stop C0800, Austin, TX, 78712, USA.
Manuel K RauschDepartment of Biomedical Engineering, The University of Texas at Austin, 107 W Dean Keeton Street Stop C0800, Austin, TX, 78712, USA.
Janet ZoldanDepartment of Biomedical Engineering, The University of Texas at Austin, 107 W Dean Keeton Street Stop C0800, Austin, TX, 78712, USA.
Hsin-Chin YehDepartment of Biomedical Engineering, The University of Texas at Austin, 107 W Dean Keeton Street Stop C0800, Austin, TX, 78712, USA.
Sapun H ParekhDepartment of Biomedical Engineering, The University of Texas at Austin, 107 W Dean Keeton Street Stop C0800, Austin, TX, 78712, USA. sparekh@utexas.edu.

Funding

Dynamic ECM-Mimicking Biomaterials for Ischemia TreatmentR01HL157829 · NHLBI · UNIVERSITY OF TEXAS AT AUSTIN · PI ZOLDAN, JANETA · 2022 to 2025
$2.3M
Developing an ultrafast fluorescence lifetime imaging ophthalmoscopy system for retinal imagingR21EY033106 · NEI · UNIVERSITY OF TEXAS AT AUSTIN · PI YEH, HSIN-CHIH · 2021 to 2022
$399k
Indian Council of Medical Research Adhoc-ID.2021-9106National Science Foundation 2146549National Science Foundation 2235455National Science Foundation 2235856National Science Foundation GRFPNEI NIH HHS R21 EY033106NIH HHS EY033106NIH HHS R01HL157829Science and Engineering Research Board SERB-SRG/2021/001886Welch Foundation F-2008-20220331
6 · The paper itself

Abstract

The extracellular matrix (ECM) is a dynamic and complex microenvironment that modulates cell behavior and cell fate. Changes in ECM composition and architecture have been correlated with development, differentiation, and disease progression in various pathologies, including breast cancer [1]. Studies have shown that aligned fibers drive a pro-metastatic microenvironment, promoting the transformation of mammary epithelial cells into invasive ductal carcinoma via the epithelial-to-mesenchymal transition (EMT) [2]. The impact of ECM orientation on breast cancer metabolism, however, is largely unknown. Here, we employ two non-invasive imaging techniques, fluorescence-lifetime imaging microscopy (FLIM) and intensity-based multiphoton microscopy, to assess the metabolic states of cancer cells cultured on ECM-mimicking nanofibers in a random and aligned orientation. By tracking the changes in the intrinsic fluorescence of nicotinamide adenine dinucleotide and flavin adenine dinucleotide, as well as expression levels of metastatic markers, we reveal how ECM fiber orientation alters cancer metabolism and EMT progression. Our study indicates that aligned cellular microenvironments play a key role in promoting metastatic phenotypes of breast cancer as evidenced by a more glycolytic metabolic signature on nanofiber scaffolds of aligned orientation compared to scaffolds of random orientation. This finding is particularly relevant for subsets of breast cancer marked by high levels of collagen remodeling (e.g. pregnancy associated breast cancer), and may serve as a platform for predicting clinical outcomes within these subsets [3-6].

Indexed as

Breast cancer microenvironmentCellular metabolismEpithelial-to-mesenchymal transition (EMT)Extracellular matrix (ECM)Fluorescence lifetime imaging microscopy (FLIM)Optical redox ratio (ORR)

Identifiers

PMID38840117
PMCPMC11151503

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.