Evidence map›Paper›PMID 38837044›Full record

ArticlePlanta2024

A versatile, rapid Agrobacterium-mediated transient expression system for functional genomics studies in cannabis seedling.

Minxuan Li, Qinqin Wu, Foqin Guo, Yuzeng Ouyang, Deyong Ao, Shijun You, Yuanyuan Liu

Abstract read
PubMed Publisher
In one paragraph

Article in Planta, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. From Model Plants toPlants (Basel, Switzerland) · 2026
    Review
  2. Reducing recalcitrance of black pepper toHorticulture research · 2026
    Article
  3. Establishment ofPlants (Basel, Switzerland) · 2025
    Article
  4. Plants (Basel, Switzerland) · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Minxuan LiHaixia Institute of Science and Technology, State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Qinqin WuHaixia Institute of Science and Technology, State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Foqin GuoHaixia Institute of Science and Technology, State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Yuzeng OuyangHaixia Institute of Science and Technology, State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Deyong AoHaixia Institute of Science and Technology, State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Shijun YouHaixia Institute of Science and Technology, State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Yuanyuan LiuHaixia Institute of Science and Technology, State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. yyliu@fafu.edu.cn.ORCID http://orcid.org/0000-0001-8107-9731

Funding

Innovative Research Group Project of the National Natural Science Foundation of China 32200226Open Project of BGI-Shenzhen BGIRSZ20220010
6 · The paper itself

Abstract

MAIN

conclusionWe have developed and optimized a rapid, versatile Agrobacterium-mediated transient expression system for cannabis seedlings that can be used in functional genomics studies of both hemp-type and drug-type cannabis. Cannabis (Cannabis sativa L.) holds great promise in the medical and food industries due to its diverse chemical composition, including specialized cannabinoids. However, the study of key genes involved in various biological processes, including secondary metabolite biosynthesis, has been hampered by the lack of efficient in vivo functional analysis methods. Here, we present a novel, short-cycle, high-efficiency transformation method for cannabis seedlings using Agrobacterium tumefaciens. We used the RUBY reporter system to monitor transformation results without the need for chemical treatments or specialized equipment. Four strains of A. tumefaciens (GV3101, EHA105, LBA4404, and AGL1) were evaluated for transformation efficiency, with LBA4404 and AGL1 showing superior performance. The versatility of the system was further demonstrated by successful transformation with GFP and GUS reporter genes. In addition, syringe infiltration was explored as an alternative to vacuum infiltration, offering simplicity and efficiency for high-throughput applications. Our method allows rapid and efficient in vivo transformation of cannabis seedlings, facilitating large-scale protein expression and high-throughput characterization studies.

Indexed as

Agrobacterium tumefaciensCannabisGenomicsSeedlingsTransformation, GeneticGenes, ReporterGreen Fluorescent ProteinsPlants, Genetically ModifiedGreen Fluorescent Proteins

Identifiers

PMID38837044

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.