HIV-1 virion lysis following centrifugation improves the sensitivity of the Fourth-Generation HIV Ag/Ab combo assay.

Allan Wandera, Kenneth Ssekatawa, Charles Drago Kato, Eliah Kwizera, Pastori Mujinya, Robert Siida

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Article in BMC research notes, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

6 authors.

Allan WanderaDepartment of Biochemistry, Faculty of Biomedical Sciences, Kampala International University, Western-Campus, P.O Box 71, Bushenyi, Uganda.

Kenneth SsekatawaDepartment of Science, Technical and Vocational Education, Makerere University, P. O. Box 7062, Kampala, Uganda. kssekatee@gmail.com.

Charles Drago KatoDepartment of Biotechnical and Diagnostic Sciences, Makerere University, P.O Box 7062, Kampala, Uganda.

Eliah KwizeraDepartment of Biochemistry, Faculty of Biomedical Sciences, Kampala International University, Western-Campus, P.O Box 71, Bushenyi, Uganda.

Pastori MujinyaDepartment of Biochemistry, Faculty of Biomedical Sciences, Kampala International University, Western-Campus, P.O Box 71, Bushenyi, Uganda.

Robert SiidaDepartment of Biochemistry, Faculty of Biomedical Sciences, Kampala International University, Western-Campus, P.O Box 71, Bushenyi, Uganda.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveFourth-generation HIV Ag/Ab Combo assay is used for HIV screening of blood for transfusion in developing countries, however, the sensitivity of the assay is questionable during the acute phase of HIV infection. Thus, the study aimed to determine the effect of combining centrifugation with HIV-1 virion lysis on the sensitivity of the fourth-generation HIV Ag/Ab combo assay.

resultsWhen the 50 HIV-1 antibody-negative samples were run on the fourth-generation HIV Ag/Ab combo assay, 8 (16%) were positive following centrifugation, 13 (26%) were positive following lysis while 25 (50%) were positive after combining centrifugation with HIV-1 virion lysis.

Indexed as

CentrifugationHIV-1HIV AntibodiesHIV InfectionsSensitivity and SpecificityVirionHIV AntigensHumansHIV AntibodiesHIV AntigensCentrifugationFourth-generation combo assayHIV-1 virion lysisSensitivity

Identifiers

PMID38835056

DOI10.1186/s13104-024-06810-y

PMCPMC11151579

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