Evidence map›Paper›PMID 38834993›Full record

ArticleMicrobial cell factories2024

Improving the enzymatic activity and stability of N-carbamoyl hydrolase using deep learning approach.

Fa Zhang, Muhammad Naeem, Bo Yu, Feixia Liu, Jiansong Ju

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbial cell factories, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Fa ZhangCollege of Life Science, Hebei Normal University, Shijiazhuang, 050024, China.
Muhammad NaeemCollege of Life Science, Hebei Normal University, Shijiazhuang, 050024, China.
Bo YuInstitute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
Feixia LiuInstitute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China. liufeixia@im.ac.cn.
Jiansong JuCollege of Life Science, Hebei Normal University, Shijiazhuang, 050024, China. jujiansong@126.com.

Funding

Funds for Central Guiding Local Science and Technology Development 236Z2801GNational Key R&D Program of China 2018YFA0901400National Natural Science Foundation of China 31971204
6 · The paper itself

Abstract

backgroundOptically active D-amino acids are widely used as intermediates in the synthesis of antibiotics, insecticides, and peptide hormones. Currently, the two-enzyme cascade reaction is the most efficient way to produce D-amino acids using enzymes DHdt and DCase, but DCase is susceptible to heat inactivation. Here, to enhance the enzymatic activity and thermal stability of DCase, a rational design software "Feitian" was developed based on k

resultsAccording to empirical design and prediction of "Feitian" software, six single-point mutants with high k

conclusionsIn this study, an efficient rational design software "Feitian" was successfully developed with a prediction accuracy of about 50% in enzymatic activity. A triple-point mutant DCase-M3 (Q4C/T212S/A302C) with enhanced enzymatic activity and thermostability was successfully obtained, which could be applied to the development of a fully enzymatic process for the industrial production of D-HPG.

Indexed as

Deep LearningEnzyme StabilityMutagenesis, Site-DirectedDeep Learning moduleD-p-hydroxyphenylglycineMolecular dynamics simulationN-carbamoyl-D-amino acid amidohydrolaseRational design

Identifiers

PMID38834993
PMCPMC11151596

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.