ArticleThe Plant cell2024
The calcium-dependent protein kinase CPK16 regulates hypoxia-induced ROS production by phosphorylating the NADPH oxidase RBOHD in Arabidopsis.
Article in The Plant cell, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- The ZmRACK1-ZmCDPK7-ZmAPX1 module regulates plant antiviral immunity.Journal of integrative plant biology · 2026Article
- Review
- Distinct processes contributing to post-submergence recovery determine leaf age-specific flood resilience in Arabidopsis.Plant physiology · 2026Article
- Phosphorylation networks as regulatory hubs in plant stress signaling: kinase dynamics, crosstalk, and network plasticity.Plant cell reports · 2026Review
- The SnRK1-RAP2.4h-PIP2 module contributes to the trade-off between growth and hypoxia tolerance in plants.Nature communications · 2026Article
- Elucidating the Role of Oxidative Stress-Associated Genes FKBP Prolyl Isomerase 5 in Osteoarthritis Development and Immunological Milieu.Biological procedures online · 2026Article
- MITOGEN-ACTIVATED PROTEIN KINASE9 fine-tunes hypoxia signaling by phosphorylating ERF-VII transcription factors in Arabidopsis.The New phytologist · 2026Article
- Transcriptome-Based Dissection of the Molecular Mechanisms Underlying Flooding Stress Responses of Eastern Cottonwood in the Floodplains of the Middle and Lower Reaches of the Yangtze River.Plants (Basel, Switzerland) · 2026Article
- Heat Shock Transcription Factor OsHsfc1a Enhances Rice Seedling Thermotolerance by Regulating OsMFT1 and Preserving Chloroplast Structure Under Heat Stress.Plant biotechnology journal · 2026Article
- Waterlogging tolerance in pigeonpea: current understanding of physiological responses, genetic resources, and genomic prospects.Frontiers in plant science · 2026Review
- A shield of phosphorylation: MPK3/6 protect STOP1 from PUB24-mediated degradation under hypoxia.The Plant cell · 2025Article
- Classification of Calcium-Dependent Protein Kinases and Their Transcriptional Response to Abiotic Stresses in HalophytePlants (Basel, Switzerland) · 2025Article
- Article
- SAL1-PAP retrograde signaling orchestrates photosynthetic and extracellular reactive oxygen species for stress responses.The Plant journal : for cell and molecular biology · 2025Article
- Harnessing Antioxidants in Cancer Therapy: Opportunities, Challenges, and Future Directions.Antioxidants (Basel, Switzerland) · 2025Review
- Principles of signal integration in combinatorial stress acclimatization.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- Reactive Oxygen Species in Plants: Metabolism, Signaling, and Oxidative Modifications.Antioxidants (Basel, Switzerland) · 2025Review
- Article
- Transcriptome, miRNA, and degradome sequencing reveal the leaf stripe (Pyrenophora graminea) resistance genes in Tibetan hulless barley.BMC plant biology · 2025Article
- Comprehensive Identification and Expression Analysis of the Multidrug and Toxic Compound Extrusion (MATE) Gene Family inPlants (Basel, Switzerland) · 2024Article
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Abstract
Reactive oxygen species (ROS) production is a key event in modulating plant responses to hypoxia and post-hypoxia reoxygenation. However, the molecular mechanism by which hypoxia-associated ROS homeostasis is controlled remains largely unknown. Here, we showed that the calcium-dependent protein kinase CPK16 regulates plant hypoxia tolerance by phosphorylating the plasma membrane-anchored NADPH oxidase respiratory burst oxidase homolog D (RBOHD) to regulate ROS production in Arabidopsis (Arabidopsis thaliana). In response to hypoxia or reoxygenation, CPK16 was activated through phosphorylation of its Ser274 residue. The cpk16 knockout mutant displayed enhanced hypoxia tolerance, whereas CPK16-overexpressing (CPK16-OE) lines showed increased sensitivity to hypoxic stress. In agreement with these observations, hypoxia and reoxygenation both induced ROS accumulation in the rosettes of CPK16-OEs more strongly than in the rosettes of the cpk16-1 mutant or the wild type. Moreover, CPK16 interacted with and phosphorylated the N-terminus of RBOHD at 4 serine residues (Ser133, Ser148, Ser163, and Ser347) that were necessary for hypoxia- and reoxygenation-induced ROS accumulation. Furthermore, the hypoxia-tolerant phenotype of cpk16-1 was fully abolished in the cpk16 rbohd double mutant. Thus, we have uncovered a regulatory mechanism by which the CPK16-RBOHD module shapes the ROS production during hypoxia and reoxygenation in Arabidopsis.
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