Evidence map›Paper›PMID 38832814›Full record

ArticleSoft matter2024

Heterogeneous distribution of kinesin-streptavidin complexes revealed by mass photometry.

Jing Xu, Nathaniel J S Brown, Yeonee Seol, Keir C Neuman

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Soft matter, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

4 authors.

Jing XuDepartment of Physics, University of California, Merced, CA 95343, USA. jxu8@ucmerced.edu.ORCID http://orcid.org/0000-0002-7882-9105
Nathaniel J S BrownDepartment of Quantitative and Systems Biology, University of California, Merced, CA 95343, USA.
Yeonee SeolLaboratory of Single Molecule Biophysics, National Heart, Lung and Blood Institute, NIH, Bethesda, MD 20892, USA.
Keir C NeumanLaboratory of Single Molecule Biophysics, National Heart, Lung and Blood Institute, NIH, Bethesda, MD 20892, USA.

Funding

Single-molecule measurements of DNA topology and topoisomerasesZIAHL001056 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI NEUMAN, KEIR · 2009 to 2025
$22.6M
Do Cargo Membrane Fluidity and Microdomain Formation Impact Motor Protein-Based Motility?R15GM120682 · NIGMS · UNIVERSITY OF CALIFORNIA, MERCED · PI XU, JING · 2016 to 2024
$1.4M
Intramural NIH HHS ZIA HL001056NIGMS NIH HHS R15 GM120682
6 · The paper itself

Abstract

Kinesin-streptavidin complexes are widely used in microtubule-based active-matter studies. The stoichiometry of the complexes is empirically tuned but experimentally challenging to determine. Here, mass photometry measurements reveal heterogenous distributions of kinesin-streptavidin complexes. Our binding model indicates that heterogeneity arises from both the kinesin-streptavidin mixing ratio and the kinesin-biotinylation efficiency.

Identifiers

PMID38832814
PMCPMC11254546

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.