Evidence map›Paper›PMID 38831186›Full record

ArticleIn vitro cellular & developmental biology. Animal2024

Atomic force microscopy characterization of white and beige adipocyte differentiation.

Alia Mallah, Katerina Stojkova, Ronald N Cohen, Nehal Abu-Lail, Eric M Brey, Maria A Gonzalez Porras

Abstract read
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In one paragraph

Article in In vitro cellular & developmental biology. Animal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Alia MallahDepartment of Biomedical Engineering and Chemical Engineering, University of Texas at San Antonio, AET 1.3681 UTSA Circle, San Antonio, TX, 78249, USA.
Katerina StojkovaDepartment of Biomedical Engineering and Chemical Engineering, University of Texas at San Antonio, AET 1.3681 UTSA Circle, San Antonio, TX, 78249, USA.
Ronald N CohenSection of Endocrinology, Diabetes, and Metabolism, Department of Medicine, University of Chicago, Chicago, IL, USA.
Nehal Abu-LailDepartment of Biomedical Engineering and Chemical Engineering, University of Texas at San Antonio, AET 1.3681 UTSA Circle, San Antonio, TX, 78249, USA.
Eric M BreyDepartment of Biomedical Engineering and Chemical Engineering, University of Texas at San Antonio, AET 1.3681 UTSA Circle, San Antonio, TX, 78249, USA.
Maria A Gonzalez PorrasDepartment of Biomedical Engineering and Chemical Engineering, University of Texas at San Antonio, AET 1.3681 UTSA Circle, San Antonio, TX, 78249, USA. maria.gonzalezporras@utsa.edu.ORCID http://orcid.org/0000-0001-6594-8802

Funding

The Role of Extracellular Environment in Adipocyte FateF32DK122754 · NIDDK · UNIVERSITY OF TEXAS SAN ANTONIO · PI GONZALEZ PORRAS, MARIA ALEJANDRA · 2020 to 2022
$217k
NIDDK NIH HHS F32-0DK122754
6 · The paper itself

Abstract

Adipose tissue plays an essential role in systemic metabolism with white adipose tissue (WAT) making up most of the tissue and being involved in the regulation of energy homeostasis, and brown and beige adipose tissue (BAT) exhibiting thermogenic activity. There is promise in the conversion of white adipocytes into beige ones as a therapeutic potential to control and enhance systemic metabolism, but it is difficult to maintain this transformation in vivo because we do not fully understand the mechanism of conversion. In this study, we applied atomic force microscopy (AFM) to characterize beige or white adipocytes during the process of differentiation for morphology, roughness, adhesion, and elasticity at different time points. As cells differentiated to white and beige adipocytes, they exhibited morphological changes as they lipid loaded, transitioning from flattened elongated cells to a rounded shape indicating adipogenesis. While there was an initial decrease in elasticity for both beige and white adipocytes, white adipocytes exhibited a higher elasticity than beige adipocytes at all time points. Beige and white adipogenesis exhibited a decrease in adhesion energy compared to preadipocytes, yet at day 12, white adipocytes had a significant increase in adhesion energy compared to beige adipocytes. This work shows significant differences in the mechanical properties of white vs. beige adipocytes during differentiation. Results from this study contribute to a better understanding of the differentiation of adipocytes which are vital to the therapeutic induction, engineered models, and maintenance of beige adipocytes as a potential approach for enhancing systemic metabolism.

Indexed as

Adipocytes, BeigeAdipocytes, WhiteAdipogenesisCell DifferentiationMicroscopy, Atomic ForceAnimalsCell AdhesionElasticityMiceAdipocyteAdipogenesisAdipose stem cell differentiationAtomic force microscopyBeige adipocyteBrown adipocyteObesityWhite adipocyte

Identifiers

PMID38831186

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.