Evidence map›Paper›PMID 38830987›Full record

ArticleScientific reports2024

TSWIFT, a novel method for iterative staining of embedded and mounted human brain sections.

Corey M Porter, Sarah Tabler, Sooin Choi, Matthias C Truttmann

Abstract read
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Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

4 authors.

Corey M PorterDepartment of Molecular and Integrative Physiology, University of Michigan, BSRB, 109 Zina Pitcher Place, Ann Arbor, MI, 48109, USA.
Sarah TablerDepartment of Molecular and Integrative Physiology, University of Michigan, BSRB, 109 Zina Pitcher Place, Ann Arbor, MI, 48109, USA.
Sooin ChoiDepartment of Molecular and Integrative Physiology, University of Michigan, BSRB, 109 Zina Pitcher Place, Ann Arbor, MI, 48109, USA.
Matthias C TruttmannDepartment of Molecular and Integrative Physiology, University of Michigan, BSRB, 109 Zina Pitcher Place, Ann Arbor, MI, 48109, USA. mtruttma@med.umich.edu.

Funding

Research Training in BiogerontologyT32AG000114 · NIA · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI SCOTT PLETCHER · 1985 to 2026
$12.4M
Conserved regulation of proteostasis by post-translational protein AMPylationR35GM142561 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Matthias Christof Truttmann · 2021 to 2026
$2.8M
TUMOR INDUCED IMMUNOSUPPRESSIONZ01AG000114 · NIA · AGING · PI LONGO, DAN L. · 2000 to 2007
$241k
Intramural NIH HHS Z01 AG000114NIA NIH HHS AG000114NIA NIH HHS T32 AG000114NIGMS NIH HHS 1R35GM142561NIGMS NIH HHS R35 GM142561
6 · The paper itself

Abstract

Comprehensive characterization of protein networks in mounted brain tissue represents a major challenge in brain and neurodegenerative disease research. In this study, we develop a simple staining method, called TSWIFT, to iteratively stain pre-mounted formalin fixed, paraffin embedded (FFPE) brain sections, thus enabling high-dimensional sample phenotyping. We show that TSWIFT conserves tissue architecture and allows for relabeling a single mounted FFPE sample more than 10 times, even after prolonged storage at 4 °C. Our results establish TSWIFT as an efficient method to obtain integrated high-dimensional knowledge of cellular proteomes by analyzing mounted FFPE human brain tissue.

Indexed as

BrainParaffin EmbeddingStaining and LabelingFormaldehydeHumansProteomeProteomicsTissue FixationFormaldehydeProteome

Identifiers

PMID38830987
PMCPMC11148033

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.