ArticleScientific reports2024
3D-bioprinted GelMA/gelatin/amniotic membrane extract (AME) scaffold loaded with keratinocytes, fibroblasts, and endothelial cells for skin tissue engineering.
Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Siloxane-Reinforced GelMA/Methacrylated Chitosan Hydrogels for DLP Printing.Gels (Basel, Switzerland) · 2026Article
- Early Compound Nutritional Intervention Improves Hair Follicle Development in Northwest Xizang White Cashmere Goats: An Analysis Based on Skin Multi-Omics.Animals : an open access journal from MDPI · 2026Article
- Alkylsuccinylated Bentonite-GelMA Hybrid Hydrogels for Enhanced Mechanics and Osteogenic Drug Delivery.ACS omega · 2026Article
- Harnessing the Bio-Instructive Placental Extracellular Matrix: Structural Properties, Decellularization, and Applications in Regenerative Medicine.International journal of molecular sciences · 2026Review
- Amniotic Membrane-Derived Factors in Immunomodulation and Regenerative Medicine: Current Evidence and Emerging Perspectives in Biomaterials and 3D Bioprinting.Journal of functional biomaterials · 2026Review
- Engineering vascularized 3D tissues: An optimized seeding protocol for collagen-based scaffolds.MethodsX · 2026Article
- Ethanol precipitation with cooling enables rapid purification of gelatin methacryloyl (GelMA) with increased yield and preserved solution-state organisation.Scientific reports · 2026Article
- A Multicellular 3D GelMA-Based Colorectal Cancer Model for Chemotherapeutic Responses.Macromolecular bioscience · 2026Article
- Phototunable Gelatin-Based Hydrogels as Cell Scaffolds for Modulating Cell Adhesion on Hydrogel Surfaces.ACS applied bio materials · 2026Article
- Photocrosslinkable Dexamethasone-Loaded GelMA Hydrogel for Peripheral Nerve Injury: Mechanical Behaviour and Anti-Adhesion Effect.Polymers · 2026Article
- Bio-inks for skin regeneration: phase-adaptive design, multifunctional platforms, and 4D bioprinting frontiers.Frontiers in bioengineering and biotechnology · 2026Review
- Fabrication of Patterned Composite Microneedles via Inkjet Printing for Enhanced Drug Delivery.Advanced healthcare materials · 2026Article
- The construction of the gelatin methacrylate microneedle -encapsulated newcastle disease virus and its antitumor effect in hepatocellular carcinoma.Journal of biological engineering · 2025Article
- Reconstructing the female reproductive system using 3D bioprinting in tissue engineering.Materials today. Bio · 2025Review
- Histological Processing of Scaffolds: Challenges and Solutions.Journal of functional biomaterials · 2025Review
- Extracellular-Matrix-Mimetic Hydrogels by Using Nanomaterials.International journal of molecular sciences · 2025Review
- Application of Fetal Membranes and Natural Materials for Wound and Tissue Repair.International journal of molecular sciences · 2024Review
- Biofabrication and Monitoring of a 3D Printed Skin Model for Melanoma.Advanced healthcare materials · 2024Article
- 3D bioprinting of skin equivalents: Towards functional wound healing models.Journal of tissue engineeringReview
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Gelatin-methacryloyl (GelMA) is a highly adaptable biomaterial extensively utilized in skin regeneration applications. However, it is frequently imperative to enhance its physical and biological qualities by including supplementary substances in its composition. The purpose of this study was to fabricate and characterize a bi-layered GelMA-gelatin scaffold using 3D bioprinting. The upper section of the scaffold was encompassed with keratinocytes to simulate the epidermis, while the lower section included fibroblasts and HUVEC cells to mimic the dermis. A further step involved the addition of amniotic membrane extract (AME) to the scaffold in order to promote angiogenesis. The incorporation of gelatin into GelMA was found to enhance its stability and mechanical qualities. While the Alamar blue test demonstrated that a high concentration of GelMA (20%) resulted in a decrease in cell viability, the live/dead cell staining revealed that incorporation of AME increased the quantity of viable HUVECs. Further, gelatin upregulated the expression of KRT10 in keratinocytes and VIM in fibroblasts. Additionally, the histological staining results demonstrated the formation of well-defined skin layers and the creation of extracellular matrix (ECM) in GelMA/gelatin hydrogels during a 14-day culture period. Our study showed that a 3D-bioprinted composite scaffold comprising GelMA, gelatin, and AME can be used to regenerate skin tissues.
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