Evidence map›Paper›PMID 38829426›Full record

ArticleArchives of microbiology2024

Sclerotia degradation by Trichoderma-mycoparasitic; an effective and sustainable trend in the drop lettuce disease control caused by Sclerotinia sclerotiorum.

Ali Athafah Tomah, Arif Ali Khattak, Mohammed Hamdan Aldarraji, Ali Abbas Hashim Al-Maidi, Mohamed Mohany, Salim S Al-Rejaie, Solabomi Olaitan Ogunyemi

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Article in Archives of microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Ali Athafah TomahPlant Protection, College of Agriculture, University of Misan, AL-Amarah, 62001, Iraq. ali_athafah@uomisan.edu.iq.ORCID http://orcid.org/0000-0002-9186-3367
Arif Ali KhattakCollege of Agriculture, South China Agricultural University, Guangzhou, 510642, China.ORCID http://orcid.org/0009-0000-4314-5021
Mohammed Hamdan AldarrajiPlant Protection, College of Agriculture, University of Misan, AL-Amarah, 62001, Iraq.
Ali Abbas Hashim Al-MaidiPlant Protection, College of Agriculture, University of Misan, AL-Amarah, 62001, Iraq.ORCID http://orcid.org/0000-0001-5323-4351
Mohamed MohanyDepartment of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box 55760, 11451, Riyadh, Saudi Arabia.ORCID http://orcid.org/0000-0001-7709-5253
Salim S Al-RejaieDepartment of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box 55760, 11451, Riyadh, Saudi Arabia.ORCID http://orcid.org/0000-0002-9254-1087
Solabomi Olaitan OgunyemiState Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Biotechnology, Zhejiang University, Hangzhou, China. 0622251@zju.edu.cn.ORCID http://orcid.org/0000-0002-3890-4107

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Controlling the hazard of sclerotia produced by the Sclerotinia sclerotiorum is very complex, and it is urgent to adopt an effective method that is harmonious environmentally to control the disease. Among the six isolates isolated from the rhizosphere of lettuce, the isolate HZA84 demonstrated a high activity in its antagonism towards Sclerotinia sclerotiorum in vitro, and produces siderophore. By amplification of internal transcribed spacer (ITS), translation elongation factor 1-alpha (TEF1-α), and RNA polymerase II subunit (RPB2) genes, the isolate HZA84 was identified as Trichoderma asperellum, which was confirmed by analysis of phylogenetic tree. The Scanning electron microscope monitoring detected that the isolate HZA84 spread over the sclerotial surface, thus, damaging, decomposing, and distorting the globular cells of the outer cortex of the sclerotia. The Real-time polymerase chain reaction (RT-qPCR) analysis disclosed the overexpression of two genes (chit33 and chit37) encoding the endochitinase in addition to one gene (prb1) encoding the proteinase during 4 and 8 days of the parasitism behavior of isolate HZA84 on the sclerotia surface. These enzymes aligned together in the sclerotia destruction by hyperparasitism. On the other hand, the pots trial revealed that spraying of isolate HZA84 reduced the drop disease symptoms of lettuce. The disease severity was decreased by 19.33 and the biocontrol efficiency was increased by 80.67% within the fourth week of inoculation. These findings magnify the unique role of Trichoderma in disrupting the development of plant diseases in sustainable ways.

Indexed as

AscomycotaLactucaPhylogenyPlant DiseasesAntibiosisFungal ProteinsHypocrealesRhizosphereSoil MicrobiologyTrichodermaFungal ProteinsMycoparasiticRT-qPCRSclerotiaSustainableTrichoderma asperellum

Identifiers

PMID38829426

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.