Evidence map›Paper›PMID 38828615›Full record

ArticleJournal of dental research2024

Improved Visualization of Oral Microbial Consortia.

S T Ramirez-Puebla, J L Mark Welch, G G Borisy

Abstract read
In one paragraph

Article in Journal of dental research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. From Global to Nano: A Geographical Perspective ofPathogens (Basel, Switzerland) · 2024
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

S T Ramirez-PueblaADA Forsyth Institute, Cambridge, MA, USA.ORCID 0000-0002-2700-4955
J L Mark WelchADA Forsyth Institute, Cambridge, MA, USA.ORCID 0000-0003-0696-2334
G G BorisyADA Forsyth Institute, Cambridge, MA, USA.

Funding

Spatial Organization of the Oral MicrobiomeR01DE022586 · NIDCR · MARINE BIOLOGICAL LABORATORY · PI Jessica Leigh Mark Welch · 2013 to 2026
$8.7M
Bayesian multivariate 3D spatial modeling for microbiome image analysisR01GM126257 · NIGMS · BRIGHAM AND WOMEN'S HOSPITAL · PI LEE, KYU HA, STARR, JACQUELINE R · 2021 to 2024
$2.4M
NIDCR NIH HHS R01 DE022586NIGMS NIH HHS R01 GM126257
6 · The paper itself

Abstract

Bacteria on the tongue dorsum (TD) form consortia tens to hundreds of microns in diameter organized around a core of epithelial cells. Whole-mount preparations have been instrumental in revealing their organization and specific microbial associations. However, their thickness and intricate 3-dimensional complexity present challenges for a comprehensive spatial analysis. To overcome these challenges, we employed a complementary approach: embedding in hydrophilic plastic followed by sectioning and postsectioning labeling. Samples were labeled by hybridization with multiplexed fluorescent oligonucleotide probes and visualized by spectral imaging and linear unmixing. Application of this strategy to TD biofilms improved the visualization of bacteria that were difficult to resolve in whole-mount imaging.

Indexed as

BiofilmsMicrobial ConsortiaTongueActinomycesHumansIn Situ Hybridization, FluorescenceStreptococcus salivariusbiofilmimagingmicrobial ecologymicrobiomemicroscopyplaque biofilms

Identifiers

PMID38828615
PMCPMC11653304

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.