Quasi-perfusion studies for intensified lentiviral vector production using a continuous stable producer cell line.

Dale J Stibbs, Pedro Silva Couto, Yasuhiro Takeuchi, Qasim A Rafiq, Nigel B Jackson, Andrea C M E Rayat

Abstract read

In one paragraph

Article in Molecular therapy. Methods & clinical development, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it

0cells of the map it votes in

3citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

Characterization and evaluation of a novel polylactic acid-made bioreactor for large-scale adherent cell expansion.Applied microbiology and biotechnology · 2026
Article
Impact of physical and chemical parameters on spinoculation for chimeric antigen receptor T cell manufacturing using a quality-by-design approach.Molecular therapy. Advances · 2026
Article
Impact of Serum/Xeno-Free Medium and Cytokine Supplementation on CAR-T Cell Therapy Manufacturing in Stirred Tank Bioreactors.Biotechnology journal · 2025
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Dale J StibbsDepartment of Biochemical Engineering, University College London, Bernard Katz Building, Gower Street, London WC1E 6BT, UK.

Pedro Silva CoutoDepartment of Biochemical Engineering, University College London, Bernard Katz Building, Gower Street, London WC1E 6BT, UK.

Yasuhiro TakeuchiDivision of Infection and Immunity, University College London, Cruciform Building, Gower Street, London WC1E 6BT, UK.

Qasim A RafiqDepartment of Biochemical Engineering, University College London, Bernard Katz Building, Gower Street, London WC1E 6BT, UK.

Nigel B JacksonCytiva, 5 Harbourgate Business Park, Southampton Road, Portsmouth PO6 4BQ, UK.

Andrea C M E RayatDepartment of Biochemical Engineering, University College London, Bernard Katz Building, Gower Street, London WC1E 6BT, UK.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Quasi-perfusion culture was employed to intensify lentiviral vector (LV) manufacturing using a continuous stable producer cell line in an 8-day process. Initial studies aimed to identify a scalable seeding density, with 3, 4, and 5 × 10

Indexed as

fixed-bed bioreactorlentiviral vectorprocess intensificationquasi-perfusion processingscale-upstable producer cell line

Identifiers

PMID38827249

DOI10.1016/j.omtm.2024.101264

PMCPMC11141457

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.