Evidence map›Paper›PMID 38824233›Full record

ArticleMolecular biology reports2024

Azoxymethane-induced carcinogenesis-like model of mouse intestine and mouse embryonic stem cell-derived intestinal organoids.

Hatice Burcu Şişli, Selinay Şenkal Turhan, Ezgi Bulut Okumuş, Özüm Begüm Böke, Özüm Erdoğmuş, Berke Kül, Engin Sümer, Ayşegül Doğan

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Article in Molecular biology reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Hatice Burcu Şişli *Faculty of Engineering, Genetics and Bioengineering Department, Yeditepe University, Istanbul, Turkey.
Selinay Şenkal Turhan *Faculty of Engineering, Genetics and Bioengineering Department, Yeditepe University, Istanbul, Turkey.
Ezgi Bulut OkumuşFaculty of Engineering, Genetics and Bioengineering Department, Yeditepe University, Istanbul, Turkey.
Özüm Begüm BökeFaculty of Engineering, Genetics and Bioengineering Department, Yeditepe University, Istanbul, Turkey.
Özüm ErdoğmuşFaculty of Engineering, Genetics and Bioengineering Department, Yeditepe University, Istanbul, Turkey.
Berke KülFaculty of Engineering, Genetics and Bioengineering Department, Yeditepe University, Istanbul, Turkey.
Engin SümerFaculty of Medicine, Experimental Research Center, Yeditepe University, Istanbul, Turkey.
Ayşegül DoğanFaculty of Engineering, Genetics and Bioengineering Department, Yeditepe University, Istanbul, Turkey. aysegul.dogan@yeditepe.edu.tr.ORCID http://orcid.org/0000-0003-4160-2270

Funding

TÜBA TÜBA-GEBİP 2020Yeditepe Üniversitesi HD22003
6 · The paper itself

Abstract

backgroundTumor modeling using organoids holds potential in studies of cancer development, enlightening both the intracellular and extracellular molecular mechanisms behind different cancer types, biobanking, and drug screening. Intestinal organoids can be generated in vitro using a unique type of adult stem cells which are found at the base of crypts and are characterized by their high Lgr5 expression levels. METHODS AND

resultsIn this study, we successfully established intestinal cancer organoid models by using both the BALB/c derived and mouse embryonic stem cells (mESCs)-derived intestinal organoids. In both cases, carcinogenesis-like model was developed by using azoxymethane (AOM) treatment. Carcinogenesis-like model was verified by H&E staining, immunostaining, relative mRNA expression analysis, and LC/MS analysis. The morphologic analysis demonstrated that the number of generated organoids, the number of crypts, and the intensity of the organoids were significantly augmented in AOM-treated intestinal organoids compared to non-AOM-treated ones. Relative mRNA expression data revealed that there was a significant increase in both Wnt signaling pathway-related genes and pluripotency transcription factors in the AOM-induced intestinal organoids.

conclusionWe successfully developed simple carcinogenesis-like models using mESC-based and Lgr5 + stem cell-based intestinal organoids. Intestinal organoid based carcinogenesi models might be used for personalized cancer therapy in the future.

Indexed as

AzoxymethaneCarcinogenesisMouse Embryonic Stem CellsOrganoidsWnt Signaling PathwayAnimalsDisease Models, AnimalIntestinal MucosaIntestinal NeoplasmsIntestinesMiceMice, Inbred BALB CReceptors, G-Protein-CoupledAzoxymethaneLgr5 protein, mouseReceptors, G-Protein-CoupledAOMCancerIntestinal organoidsLgr5OrganoidPluripotency

Identifiers

PMID38824233

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.