Evidence map›Paper›PMID 38823017›Full record

ArticleCell reports2024

A cis-regulatory module underlies retinal ganglion cell genesis and axonogenesis.

Kamakshi Mehta, Marwa Daghsni, Reza Raeisossadati, Zhongli Xu, Emily Davis, Abigail Naidich, Bingjie Wang, Shiyue Tao, Shaohua Pi, Wei Chen and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Kamakshi MehtaDepartment of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA.
Marwa DaghsniDepartment of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA.
Reza RaeisossadatiDepartment of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA.
Zhongli XuDepartment of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA.
Emily DavisDepartment of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA.
Abigail NaidichDepartment of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA.
Bingjie WangDepartment of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA.
Shiyue TaoDepartment of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA.
Shaohua PiDepartment of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA.
Wei ChenDepartment of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA.
Dennis KostkaDepartment of Computational & Systems Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA.
Silvia LiuDepartment of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA.
Jeffrey M GrossDepartment of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA.
Takaaki KuwajimaDepartment of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA; Louis J. Fox Center for Vision Restoration, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA.
Issam AldiriDepartment of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA; Department of Computational & Systems Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA; Louis J. Fox Center for Vision Restoration, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA. Electronic address: aldirii@pitt.edu.

Funding

Virus Production and Manipulation of Protein/Gene Expression ModuleP30EY008098 · NEI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI Yuanyuan Chen · 1989 to 2026
$17.8M
Epigenetic basis of retinal cell fate determinationR01EY030861 · NEI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI AL DIRI, ISSAM · 2020 to 2024
$1.9M
DNA Methylation and Hydroxymethylation During Retinal Development and Stem Cell MaintenanceR01EY029031 · NEI · UNIVERSITY OF TEXAS AT AUSTIN · PI GROSS, JEFFREY · 2018 to 2022
$1.9M
NEI NIH HHS P30 EY008098NEI NIH HHS R01 EY029031NEI NIH HHS R01 EY030861
6 · The paper itself

Abstract

Atoh7 is transiently expressed in retinal progenitor cells (RPCs) and is required for retinal ganglion cell (RGC) differentiation. In humans, a deletion in a distal non-coding regulatory region upstream of ATOH7 is associated with optic nerve atrophy and blindness. Here, we functionally interrogate the significance of the Atoh7 regulatory landscape to retinogenesis in mice. Deletion of the Atoh7 enhancer structure leads to RGC deficiency, optic nerve hypoplasia, and retinal blood vascular abnormalities, phenocopying inactivation of Atoh7. Further, loss of the Atoh7 remote enhancer impacts ipsilaterally projecting RGCs and disrupts proper axonal projections to the visual thalamus. Deletion of the Atoh7 remote enhancer is also associated with the dysregulation of axonogenesis genes, including the derepression of the axon repulsive cue Robo3. Our data provide insights into how Atoh7 enhancer elements function to promote RGC development and optic nerve formation and highlight a key role of Atoh7 in the transcriptional control of axon guidance molecules.

Indexed as

AxonsBasic Helix-Loop-Helix ProteinsEnhancer Elements, GeneticRetinal Ganglion CellsAnimalsCell DifferentiationGene Expression Regulation, DevelopmentalMiceMice, Inbred C57BLNerve Tissue ProteinsNeurogenesisOptic NerveReceptors, Cell SurfaceReceptors, ImmunologicRetinaRoundabout ProteinsAtoh7 protein, mouseBasic Helix-Loop-Helix ProteinsNerve Tissue ProteinsReceptors, Cell SurfaceReceptors, ImmunologicRobo3 protein, mouseRoundabout Proteinscell fatechromatinCP: Developmental biologyCP: Neuroscienceretinal developmentretinotectal maptranscription factor

Identifiers

PMID38823017
PMCPMC11238474

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.