Evidence map›Paper›PMID 38822881›Full record

ArticleMolecular biology reports2024

Hsa_circ_0096157 silencing suppresses autophagy and reduces cisplatin resistance in non-small cell lung cancer by weakening the Nrf2/ARE signaling pathway.

Huasong Lu, Jinliang Kong, Shuangqi Cai, Hong Huang, Jing Luo, Lihua Liu

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Article in Molecular biology reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers, 1 of them a synthesis that pooled it.

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5citing papers in PubMed, 1 pooled it
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1 · What the graph read from it

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3 · Its place in the literature

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5 citing papers in PubMed, 1 synthesis or guideline pooled it.

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Huasong LuDepartment of Pulmonary and Critical Care Medicine, the First Affiliated Hospital of Guangxi Medical University, No. 6, Shuangyong Road, Nanning, 530021, People's Republic of China.
Jinliang KongDepartment of Pulmonary and Critical Care Medicine, the First Affiliated Hospital of Guangxi Medical University, No. 6, Shuangyong Road, Nanning, 530021, People's Republic of China.
Shuangqi CaiDepartment of Pulmonary and Critical Care Medicine, the First Affiliated Hospital of Guangxi Medical University, No. 6, Shuangyong Road, Nanning, 530021, People's Republic of China.
Hong HuangDepartment of Pulmonary and Critical Care Medicine, the First Affiliated Hospital of Guangxi Medical University, No. 6, Shuangyong Road, Nanning, 530021, People's Republic of China.
Jing LuoDepartment of Pulmonary and Critical Care Medicine, the First Affiliated Hospital of Guangxi Medical University, No. 6, Shuangyong Road, Nanning, 530021, People's Republic of China.
Lihua LiuDepartment of Pulmonary and Critical Care Medicine, the First Affiliated Hospital of Guangxi Medical University, No. 6, Shuangyong Road, Nanning, 530021, People's Republic of China. lhlhliu@163.com.

Funding

National Natural Science Foundation of China 82260576Natural Science Foundation of Guangxi 101112535008 and 2020JJA140405
6 · The paper itself

Abstract

backgroundNon-small cell lung cancer (NSCLC) is the leading cause of cancer morbidity and mortality worldwide, and new diagnostic markers are urgently needed. We aimed to investigate the mechanism by which hsa_circ_0096157 regulates autophagy and cisplatin (DDP) resistance in NSCLC.

methodsA549 cells were treated with DDP (0 μg/mL or 3 μg/mL). Then, the autophagy activator rapamycin (200 nm) was applied to the A549/DDP cells. Moreover, hsa_circ_0096157 and Nrf2 were knocked down, and Nrf2 was overexpressed in A549/DDP cells. The expression of Hsa_circ_0096157, the Nrf2/ARE pathway-related factors Nrf2, HO-1, and NQO1, and the autophagy-related factors LC3, Beclin-1, and p62 was evaluated by qRT‒PCR or western blotting. Autophagosomes were detected through TEM. An MTS assay was utilized to measure cell proliferation. The associated miRNA levels were also tested by qRT‒PCR.

resultsDDP (3 μg/mL) promoted hsa_circ_0096157, LC3 II/I, and Beclin-1 expression and decreased p62 expression. Knocking down hsa_circ_0096157 resulted in the downregulation of LC3 II/I and Beclin-1 expression, upregulation of p62 expression, and decreased proliferation. Rapamycin reversed the effect of interfering with hsa_circ_0096157. Keap1 expression was lower, and Nrf2, HO-1, and NQO1 expression was greater in the A549/DDP group than in the A549 group. HO-1 expression was repressed after Nrf2 interference. In addition, activation of the Nrf2/ARE pathway promoted autophagy in A549/DDP cells. Moreover, hsa_circ_0096157 activated the Nrf2/ARE pathway. The silencing of hsa_circ_0096157 reduced Nrf2 expression by releasing miR-142-5p or miR-548n. Finally, we found that hsa_circ_0096157 promoted A549/DDP cell autophagy by activating the Nrf2/ARE pathway.

conclusionKnockdown of hsa_circ_0096157 inhibits autophagy and DDP resistance in NSCLC cells by downregulating the Nrf2/ARE signaling pathway.

Indexed as

AutophagyCarcinoma, Non-Small-Cell LungCisplatinDrug Resistance, NeoplasmGene Expression Regulation, NeoplasticLung NeoplasmsNF-E2-Related Factor 2Signal TransductionA549 CellsAntineoplastic AgentsAntioxidant Response ElementsCell Line, TumorCell ProliferationHeme Oxygenase-1HumansMicroRNAsAntineoplastic AgentsCisplatinHeme Oxygenase-1MicroRNAsNFE2L2 protein, humanNF-E2-Related Factor 2AutophagyCisplatinHsa_circ_0096157Non-small cell lung cancerNrf2/ARE signaling pathway

Identifiers

PMID38822881

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.