Evidence map›Paper›PMID 38822400›Full record

ArticleVeterinary research2024

Interferon-gamma producing CD4

Hakan Bulun, Philip S Bridger, Simone Schillinger, Ömer Akineden, Stefanie A Barth, Marta Fischer, Manfred Henrich, Torsten Seeger, Klaus Doll, Michael Bülte and 2 more

Abstract read
In one paragraph

Article in Veterinary research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Hakan BulunInstitute for Hygiene & Infectious Diseases of Animals, Justus Liebig University Giessen, Giessen, Germany.
Philip S BridgerInstitute for Hygiene & Infectious Diseases of Animals, Justus Liebig University Giessen, Giessen, Germany.
Simone SchillingerInstitute for Hygiene & Infectious Diseases of Animals, Justus Liebig University Giessen, Giessen, Germany.
Ömer AkinedenInstitute for Veterinary Food Sciences, Justus Liebig University Giessen, Giessen, Germany.
Stefanie A BarthInstitute for Hygiene & Infectious Diseases of Animals, Justus Liebig University Giessen, Giessen, Germany.
Marta FischerInstitute for Veterinary Food Sciences, Justus Liebig University Giessen, Giessen, Germany.
Manfred HenrichDepartment for Veterinary Pathology, Justus Liebig University Giessen, Giessen, Germany.
Torsten SeegerClinic for Ruminants & Swine, University of Giessen, Giessen, Germany.
Klaus DollClinic for Ruminants & Swine, University of Giessen, Giessen, Germany.
Michael BülteInstitute for Veterinary Food Sciences, Justus Liebig University Giessen, Giessen, Germany.
Rolf BauerfeindInstitute for Hygiene & Infectious Diseases of Animals, Justus Liebig University Giessen, Giessen, Germany.
Christian MengeInstitute for Hygiene & Infectious Diseases of Animals, Justus Liebig University Giessen, Giessen, Germany. christian.menge@fli.de.ORCID http://orcid.org/0009-0007-0049-1371

Funding

Bundesanstalt für Landwirtschaft und Ernährung 28-1-32.006-06
6 · The paper itself

Abstract

Current diagnostic methods for Johne's disease in cattle allow reliable detection of infections with Mycobacterium avium ssp. paratuberculosis (MAP) not before animals are 2 years of age. Applying a flow cytometry-based approach (FCA) to quantify a MAP-specific interferon-gamma (IFN-γ) response in T cell subsets, the present study sought to monitor the kinetics of the cell-mediated immune response in experimentally infected calves. Six MAP-negative calves and six calves, orally inoculated with MAP at 10 days of age, were sampled every 4 weeks for 52 weeks post-inoculation (wpi). Peripheral blood mononuclear cells (PBMC) were stimulated with either purified protein derivatives (PPD) or whole cell sonicates derived from MAP (WCSj), M. avium ssp. avium or M. phlei for 6 days followed by labeling of intracellular IFN-γ in CD4

Indexed as

Cattle DiseasesCD4-Positive T-LymphocytesFlow CytometryInterferon-gammaMycobacterium avium subsp. paratuberculosisParatuberculosisAnimalsBiomarkersCattleBiomarkersInterferon-gammaflow cytometryIFN-γJohne’s diseaseM. avium ssp. paratuberculosisT cell

Identifiers

PMID38822400
PMCPMC11143577

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.