Evidence map›Paper›PMID 38818480›Full record

ArticleRegenerative therapy2024

METTL3 promotes the osteogenic differentiation of periosteum-derived MSCs via regulation of the HOXD8/ITGA5 axis in congenital pseudarthrosis.

Weihua Ye, Zheng Liu, Yaoxi Liu, Han Xiao, Qian Tan, An Yan, Guanghui Zhu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Regenerative therapy, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Review
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  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Weihua YeOrthopedic Department, Hunan Provincial Key Laboratory of Pediatric Orthopedics, Hunan Children's Hospital, Children's Hospital Affiliated to Xiangya Medical College of Central South University, 86# Ziyuan Road, Changsha, Hunan 410007, China.
Zheng LiuOrthopedic Department, Hunan Provincial Key Laboratory of Pediatric Orthopedics, Hunan Children's Hospital, Children's Hospital Affiliated to Xiangya Medical College of Central South University, 86# Ziyuan Road, Changsha, Hunan 410007, China.
Yaoxi LiuOrthopedic Department, Hunan Provincial Key Laboratory of Pediatric Orthopedics, Hunan Children's Hospital, Children's Hospital Affiliated to Xiangya Medical College of Central South University, 86# Ziyuan Road, Changsha, Hunan 410007, China.
Han XiaoOrthopedic Department, Hunan Provincial Key Laboratory of Pediatric Orthopedics, Hunan Children's Hospital, Children's Hospital Affiliated to Xiangya Medical College of Central South University, 86# Ziyuan Road, Changsha, Hunan 410007, China.
Qian TanOrthopedic Department, Hunan Provincial Key Laboratory of Pediatric Orthopedics, Hunan Children's Hospital, Children's Hospital Affiliated to Xiangya Medical College of Central South University, 86# Ziyuan Road, Changsha, Hunan 410007, China.
An YanOrthopedic Department, Hunan Provincial Key Laboratory of Pediatric Orthopedics, Hunan Children's Hospital, Children's Hospital Affiliated to Xiangya Medical College of Central South University, 86# Ziyuan Road, Changsha, Hunan 410007, China.
Guanghui ZhuOrthopedic Department, Hunan Provincial Key Laboratory of Pediatric Orthopedics, Hunan Children's Hospital, Children's Hospital Affiliated to Xiangya Medical College of Central South University, 86# Ziyuan Road, Changsha, Hunan 410007, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Congenital pseudarthrosis of the tibia (CPT) is a dominant health challenge in pediatric orthopedics. The essential process in the development of CPT is the limited capacity of mesenchymal stem cells (MSCs) derived from CPT to undergo osteogenic differentiation. Our research aimed to elucidate the role and mechanism of methyltransferase-like 3 (METTL3) in the osteogenic differentiation process of CPT MSCs. Methods: The osteogenic differentiation medium was used to culture MSCs, and the detection of osteogenic differentiation was performed using Alizarin Red S and alkaline phosphatase (ALP) assays. Gene or protein expression was assessed by quantitative real-time polymerase chain reaction (qRT-PCR), Western blot, or immunofluorescence (IF) staining. The m Results: METTL3 overexpression enhanced CPT MSCs' osteogenic differentiation. METTL3 stabilized the HOXD8 in an m Conclusion: METTL3 promoted osteogenic differentiation via modulating the HOXD8/ITGA5 axis in CPT MSCs.

Indexed as

CPT MSCsHOXD8ITGA5METTL3Osteogenic differentiation

Identifiers

PMID38818480
PMCPMC11137358

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.