Evidence map›Paper›PMID 38817321›Full record

ArticleChemical & biomedical imaging2024

Multivariate Analysis of Individual Bacterial Outer Membrane Vesicles Using Fluorescence Microscopy.

Aarshi N Singh, Justin B Nice, Meishan Wu, Angela C Brown, Nathan J Wittenberg

Abstract read
In one paragraph

Article in Chemical & biomedical imaging, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Review
  2. E-cigarette liquids alter the biogenesis and properties ofFrontiers in cellular and infection microbiology · 2026
    Article
  3. Article
  4. Article
  5. Article
  6. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Aarshi N SinghDepartment of Chemistry, Lehigh University, Bethlehem, Pennsylvania 18015, United States.ORCID https://orcid.org/0000-0002-5888-4449
Justin B NiceDepartment of Chemical and Biomolecular Engineering, Lehigh University, Bethlehem, Pennsylvania 18015, United States.
Meishan WuDepartment of Chemical and Biomolecular Engineering, Lehigh University, Bethlehem, Pennsylvania 18015, United States.
Angela C BrownDepartment of Chemical and Biomolecular Engineering, Lehigh University, Bethlehem, Pennsylvania 18015, United States.ORCID https://orcid.org/0000-0003-1038-8216
Nathan J WittenbergDepartment of Chemistry, Lehigh University, Bethlehem, Pennsylvania 18015, United States.ORCID https://orcid.org/0000-0001-9196-1867

Funding

Elucidating the membrane properties regulating antimicrobial peptidebinding to bacterial vesiclesR15GM152918 · NIGMS · LEHIGH UNIVERSITY · PI WITTENBERG, NATHAN J. · 2023 to 2023
$457k
Mechanism of LtxA Enrichment in A. Actinomycetemcomitans Outer Membrane VesiclesR21DE027769 · NIDCR · LEHIGH UNIVERSITY · PI BROWN, ANGELA C. · 2018 to 2019
$426k
Microfluidic nanoarrays for high-throughput analysis of biological nanostructuresR21GM134414 · NIGMS · LEHIGH UNIVERSITY · PI WITTENBERG, NATHAN J. · 2019 to 2020
$409k
Controlled antibiotic delivery vehicle for treatment of aggressiveperiodontitisR21DE032153 · NIDCR · LEHIGH UNIVERSITY · PI BROWN, ANGELA C. · 2023 to 2024
$408k
NIDCR NIH HHS R21 DE027769NIDCR NIH HHS R21 DE032153NIGMS NIH HHS R15 GM152918NIGMS NIH HHS R21 GM134414
6 · The paper itself

Abstract

Gram-negative bacteria produce outer membrane vesicles (OMVs) that play a critical role in cell-cell communication and virulence. OMVs have emerged as promising therapeutic agents for various biological applications such as vaccines and targeted drug delivery. However, the full potential of OMVs is currently constrained by inherent heterogeneities, such as size and cargo differences, and traditional ensemble assays are limited in their ability to reveal OMV heterogeneity. To overcome this issue, we devised an innovative approach enabling the identification of various characteristics of individual OMVs. This method, employing fluorescence microscopy, facilitates the detection of variations in size and surface markers. To demonstrate our method, we utilize the oral bacterium

Identifiers

PMID38817321
PMCPMC11134603

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.