ArticleNature communications2024
Targeted suppression of siRNA biogenesis in Arabidopsis pollen promotes triploid seed viability.
Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Presence and function of small RNAs during plant reproduction.RNA biology · 2026Review
- Review
- New molecular players: siRNA expression and gene regulation in Hordeum vulgare L. ageing seeds after germination.BMC genomics · 2026Article
- Cell-type-specific ablation of siRNAs by Arabidopsis RTL1 reveals a role of phloem companion cells in systemic post-transcriptional gene silencing.Nucleic acids research · 2026Article
- Three decades of mobile RNA silencing within plants: what have we learnt?Journal of experimental botany · 2026Review
- Decoding hybridization barriers: the molecular and genetic orchestration of the triploid block in Arabidopsis thaliana.BMC plant biology · 2025Review
- Pseudouridine guides germline small RNA transport and epigenetic inheritance.Nature structural & molecular biology · 2025Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
In plants, small-interfering RNAs (siRNAs) mediate epigenetic silencing via the RNA-directed DNA methylation (RdDM) pathway, which is particularly prominent during reproduction and seed development. However, there is limited understanding of the origins and dynamics of reproductive siRNAs acting in different cellular and developmental contexts. Here, we used the RNaseIII-like protein RTL1 to suppress siRNA biogenesis in Arabidopsis pollen, and found distinct siRNA subsets produced during pollen development. We demonstrate that RTL1 expression in the late microspore and vegetative cell strongly impairs epigenetic silencing, and resembles RdDM mutants in their ability to bypass interploidy hybridization barriers in the seed. However, germline-specific RTL1 expression did not impact transgenerational inheritance of triploid seed lethality. These results reveal the existence of multiple siRNA subsets accumulated in mature pollen, and suggest that mobile siRNAs involved in the triploid block are produced in germline precursor cells after meiosis, or in the vegetative cell during pollen mitosis.
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Registered trials
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