ArticleJournal of food protection2024
Sources and Prevalence of Cyclospora cayetanensis in Southeastern U.S. Growing Environments.
Article in Journal of food protection, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed.
- SenescentMicroorganisms · 2026Article
- Molecular detection of Cyclospora cayetanensis in fresh produce and irrigation water in peri-urban settings: a cross-sectional study of the environmental-agricultural interface.BMC microbiology · 2026Article
- Microbial Contamination Risks From Adjacent and Nearby Land: Evidence and Implications for Produce Safety.Journal of food science · 2026Review
- Optimization of the Mit1C real-time PCR assay using Bio-Rad CFX96 for the detection of Cyclospora cayetanensis in agricultural waters.PloS one · 2026Article
- Evaluation of nucleic acid extraction methods for recovery of Cyclospora cayetanensis, Salmonella enterica, and murine norovirus from water and sludge.Journal of microbiological methods · 2025Article
- Review
- Assessing the sequencing success and analytical specificity of a targeted amplicon deep sequencing workflow for genotyping the foodborne parasiteJournal of clinical microbiology · 2025Article
- Is Parasitic Contamination of Soil in the Southern United States Related to Poverty and Does It Represent a Human Health Threat? A Perspective.The American journal of tropical medicine and hygiene · 2025Article
- Performance of Conditional Random Forest and Regression Models at Predicting Human Fecal Contamination of Produce Irrigation Ponds in the Southeastern United States.ACS ES&T water · 2024Article
- Sources and Prevalence of Cyclospora cayetanensis in Southeastern U.S. Growing Environments.Journal of food protection · 2024Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Recent cyclosporiasis outbreaks associated with fresh produce grown in the United States highlight the need to better understand Cyclospora cayetanensis prevalence in U.S. agricultural environments. In this study, C. cayetanensis occurrence was assessed in municipal wastewater sludge, on-farm portable toilets, irrigation pond water, and spent packing house dump tank water in a Southeastern Georgia growing region over two years. Detection of the C. cayetanensis 18S rRNA qPCR gene target in pond samples was 0%, 28%, and 42% (N = 217) depending on the detection definition used, and ≤1% in dump tank samples (N = 46). However, no qPCR detections were confirmed by sequencing, suggesting false detection occurred due to cross-reactions. C. cayetanensis qPCR detections were confirmed in 9% of wastewater sludge samples (N = 76). The human-specific fecal markers HF183 and crAssphage were detected in 33% and 6% of pond samples, respectively, and 4% and 0% of dump tank samples, respectively. Despite community Cyclospora shedding and evidence of human fecal contamination in irrigation water, there was no correlation between C. cayetanensis and HF183 qPCR detections, further supporting that 18S gene target qPCR amplifications were due to cross-reactions. When evaluating C. cayetanensis qPCR environmental detection data, the impact of assay specificity and detection criteria should be considered. Moreover, additional sequence-based testing may be needed to appropriately interpret Cyclospora qPCR environmental data.
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Registered trials
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