Evidence map›Paper›PMID 38815793›Full record

ArticleExperimental eye research2024

Non-canonical Wnt pathway expression in the developing mouse and human retina.

Rosanna C Campos, Kate Matsunaga, Mark W Reid, G Esteban Fernandez, Kayla Stepanian, Sumitha P Bharathan, Meng Li, Matthew E Thornton, Brendan H Grubbs, Aaron Nagiel

Abstract read
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In one paragraph

Article in Experimental eye research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Rosanna C CamposThe Vision Center, Department of Surgery, Children's Hospital Los Angeles, Los Angeles, CA, USA; Department of Development, Stem Cells and Regenerative Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
Kate MatsunagaKeck School of Medicine, University of Southern California, Los Angeles, CA, 90033, USA.
Mark W ReidThe Vision Center, Department of Surgery, Children's Hospital Los Angeles, Los Angeles, CA, USA.
G Esteban FernandezThe Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, CA, USA.
Kayla StepanianThe Vision Center, Department of Surgery, Children's Hospital Los Angeles, Los Angeles, CA, USA.
Sumitha P BharathanThe Vision Center, Department of Surgery, Children's Hospital Los Angeles, Los Angeles, CA, USA; The Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, CA, USA.
Meng LiUSC Libraries Bioinformatics Services, University of Southern California, Los Angeles, CA, USA.
Matthew E ThorntonMaternal-Fetal Medicine Division, Department of Obstetrics and Gynecology, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
Brendan H GrubbsMaternal-Fetal Medicine Division, Department of Obstetrics and Gynecology, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
Aaron NagielThe Vision Center, Department of Surgery, Children's Hospital Los Angeles, Los Angeles, CA, USA; The Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, CA, USA; Roski Eye Institute, Department of Ophthalmology, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA. Electronic address: anagiel@chla.usc.edu.

Funding

Development and Maintenance of the Human Photoreceptor-Bipolar Cell Synapse.K08EY030924 · NEI · CHILDREN'S HOSPITAL OF LOS ANGELES · PI NAGIEL, AARON · 2020 to 2024
$1.1M
NEI NIH HHS K08 EY030924
6 · The paper itself

Abstract

The non-canonical Wnt pathway is an evolutionarily conserved pathway essential for tissue patterning and development across species and tissues. In mammals, this pathway plays a role in neuronal migration, dendritogenesis, axon growth, and synapse formation. However, its role in development and synaptogenesis of the human retina remains less established. In order to address this knowledge gap, we analyzed publicly available single-cell RNA sequencing (scRNAseq) datasets for mouse retina, human retina, and human retinal organoids over multiple developmental time points during outer retinal maturation. We identified ligands, receptors, and mediator genes with a putative role in retinal development, including those with novel or species-specific expression, and validated this expression using fluorescence in situ hybridization (FISH). By quantifying outer nuclear layer (ONL) versus inner nuclear layer (INL) expression, we provide evidence for the differential expression of certain non-canonical Wnt signaling components in the developing mouse and human retina during outer plexiform layer (OPL) development. Importantly, we identified distinct expression patterns of mouse and human FZD3 and WNT10A, as well as previously undescribed expression, such as for mouse Wnt2b in Chat

Indexed as

Gene Expression Regulation, DevelopmentalRetinaWnt Signaling PathwayAnimalsHumansIn Situ Hybridization, FluorescenceMiceMice, Inbred C57BLOrganoidsFzd3Human retinal organoidsIn situ hybridizationNon-canonical wntVangl2Wn2bWnt10a

Identifiers

PMID38815793
PMCPMC11179970

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.