Evidence map›Paper›PMID 38814393›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Generation and Characterization of In Vitro Transcribed mRNA.

Cristina Poveda, Yi-Lin Chen, Ulrich Strych

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Cristina PovedaBaylor College of Medicine, Department of Pediatrics, Division of Pediatric Tropical Medicine, Houston, TX, USA.
Yi-Lin ChenTexas Children's Hospital Center for Vaccine Development, Houston, TX, USA.
Ulrich StrychBaylor College of Medicine, Department of Pediatrics, Division of Pediatric Tropical Medicine, Houston, TX, USA. strych@bcm.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Here we describe the in vitro preparation of mRNA from DNA templates, including setting up the transcription reaction, mRNA capping, and mRNA labeling. We then describe methods used for mRNA characterization, including UV and fluorescence spectrophotometry, as well as gel electrophoresis. Moreover, characterization of the in vitro transcribed RNA using the Bioanalyzer instrument is described, allowing a higher resolution analysis of the target molecules. For the in vitro testing of the mRNA molecules, we include protocols for the transfection of various primary cell cultures and the confirmation of translation by intracellular staining and western blotting.

Indexed as

RNA, MessengerTranscription, GeneticAnimalsDNAHumansRNA CapsTransfectionDNARNA CapsRNA, MessengerFlow cytometryFluorescence spectrophotometryImmunofluorescence microscopyIntracellular stainingIn vitro transcriptionLipid nanoparticlesLNPsmRNA cappingUltraviolet spectrophotometryWestern blotting

Identifiers

PMID38814393

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.