ArticlePractical laboratory medicine2024
HPLC and flow cytometry combined approach for HbF analysis in fetomaternal haemorrhage evaluation.
Article in Practical laboratory medicine, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Severe Fetal Anaemia Secondary to Fetomaternal Haemorrhage: A Case Report Exploring Aetiologies and Diagnostics.Cureus · 2026Article
- Overview of detection methods of fetomaternal haemorrhage.Frontiers in physiology · 2025Review
- Estimation of fetomaternal haemorrhage using flow cytometry in antenatal care patients at an tertiary care hospital in Western Maharashtra.Medical journal, Armed Forces IndiaArticle
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Authors and funding
17 authors.
Funding
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Abstract
Introduction: Recently, a flow cytometric (FC) based test has been developed for detection of circulating fetal cells to replace the less accurate and reproducible Kleihauer-Betke test.FC test is easier to perform, it can distinguish the origin of fetal cells, but it is expensive and available in highly specialized laboratories. We evaluated the introduction of high-performance liquid chromatography (HPLC) approach as initial screening to identify patients who need an additional FC test to better discriminate the nature of haemoglobin-F (HbF) positive cells. Methods: Blood samples from 130 pregnant women suspected to have fetomaternal haemorrhage were analysed with HPLC and FC methods. The cut-off for HbF HPLC concentration was calculated. Statistical analyses for the evaluation of HPLC as a screening method were performed. The positivity cut-off of HbF to be used as decision-making value to continue the investigation was calculated. Results: An excellent agreement (R Conclusions: This study provides a new FMH investigation approach that possibly leads to a reduction in times and costs of the analysis.
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