Evidence map›Paper›PMID 38811698›Full record

ArticleScientific reports2024

A targeted fluorescent nanosensor for ratiometric pH sensing at the cell surface.

Charlotte Kromer, Aaron Katz, Ines Feldmann, Peter Laux, Andreas Luch, Harald R Tschiche

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Charlotte KromerProduct Materials and Nanotechnology, Department Chemical and Product Safety, German Federal Institute for Risk Assessment, Max-Dohrn-Str. 8-10, 10589, Berlin, Germany. Charlotte.Kromer@bfr.bund.de.ORCID 0000-0003-4823-4714
Aaron KatzProduct Materials and Nanotechnology, Department Chemical and Product Safety, German Federal Institute for Risk Assessment, Max-Dohrn-Str. 8-10, 10589, Berlin, Germany.ORCID 0009-0002-5681-847X
Ines FeldmannMaterial-Microbiome Interactions, Department Materials and the Environment, Federal Institute for Materials Research and Testing, Berlin, Germany.ORCID 0000-0003-2650-9270
Peter LauxProduct Materials and Nanotechnology, Department Chemical and Product Safety, German Federal Institute for Risk Assessment, Max-Dohrn-Str. 8-10, 10589, Berlin, Germany.ORCID 0000-0002-0351-3392
Andreas LuchProduct Materials and Nanotechnology, Department Chemical and Product Safety, German Federal Institute for Risk Assessment, Max-Dohrn-Str. 8-10, 10589, Berlin, Germany.ORCID 0000-0002-5866-901X
Harald R TschicheProduct Materials and Nanotechnology, Department Chemical and Product Safety, German Federal Institute for Risk Assessment, Max-Dohrn-Str. 8-10, 10589, Berlin, Germany.ORCID 0000-0002-6766-9804

Funding

German Federal Institute for Risk Assessment, Germany SFP 1322-721
6 · The paper itself

Abstract

The correlation between altered extracellular pH and various pathological conditions, including cancer, inflammation and metabolic disorders, is well known. Bulk pH measurements cannot report the extracellular pH value at the cell surface. However, there is a limited number of suitable tools for measuring the extracellular pH of cells with high spatial resolution, and none of them are commonly used in laboratories around the world. In this study, a versatile ratiometric nanosensor for the measurement of extracellular pH was developed. The nanosensor consists of biocompatible polystyrene nanoparticles loaded with the pH-inert reference dye Nile red and is surface functionalized with a pH-responsive fluorescein dye. Equipped with a targeting moiety, the nanosensor can adhere to cell membranes, allowing direct measurement of extracellular pH at the cell surface. The nanosensor exhibits a sensitive ratiometric pH response within the range of 5.5-9.0, with a calculated pKa of 7.47. This range optimally covers the extracellular pH (pH

Indexed as

Fluorescent DyesNanoparticlesBiosensing TechniquesCell MembraneHumansHydrogen-Ion ConcentrationOxazinesPolystyrenesFluorescent Dyesnile redOxazinesPolystyrenesExtracellular pHNanoparticlesNanosensorpHpHepH sensorTargeting

Identifiers

PMID38811698
PMCPMC11137054

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.