ArticleNature communications2024
Germline Cas9 promoters with improved performance for homing gene drive.
Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- The nanosd integral gene drive enables population modification of the malaria vector Anopheles gambiae.G3 (Bethesda, Md.) · 2026Article
- Finding the perfect promoter for Cas9 in homing gene drives using single cell transcriptome data.Nature communications · 2026Article
- Self-limiting population suppression gene drive design in the West Nile vector mosquito, Culex quinquefasciatus.Nature communications · 2026Article
- A simulation-based deep learning framework for spatially explicit malaria modeling of CRISPR suppression gene drive mosquitoes.iScience · 2026Article
- Experimental and modeling demonstration of stronger population suppression by gene drive targeting doublesex from dominant female-sterile resistance alleles.Nature communications · 2026Article
- Genetic engineering for SIT application: a fruit fly-focused review.Insect science · 2026Review
- Assessing target genes for homing suppression gene drive.The EMBO journal · 2026Article
- Strategies to improve the efficiency of homing gene drives with multiplexed gRNAs.BMC biology · 2025Article
- Modeling Control of Invasive Fire Ants by Gene Drive.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Synthetic homing endonuclease gene drives to revolutionise Aedes aegypti biocontrol - game changer or pipe dream?Current opinion in insect science · 2025Review
- Performance of two low-threshold population replacement gene drives in cage populations of the yellow fever mosquito, Aedes aegypti.PLoS genetics · 2025Article
- A Comparative Assessment of Self-limiting Genetic Control Strategies for Population Suppression.Molecular biology and evolution · 2025Article
- Controlling the frequency dynamics of homing gene drives for intermediate outcomes.G3 (Bethesda, Md.) · 2025Article
- Gene drive-based population suppression in the malaria vector Anopheles stephensi.Nature communications · 2025Article
- Improving the suppressive power of homing gene drive by co-targeting a distant-site female fertility gene.Nature communications · 2024Article
- Population suppression by release of insects carrying a dominant sterile homing gene drive targeting doublesex in Drosophila.Nature communications · 2024Article
- Population suppression with dominant female-lethal alleles is boosted by homing gene drive.BMC biology · 2024Article
- Population dynamics in spatial suppression gene drive models and the effect of resistance, density dependence, and life history.bioRxiv : the preprint server for biology · 2024Article
- A small-molecule approach to restore female sterility phenotype targeted by a homing suppression gene drive in the fruit pest Drosophila suzukii.PLoS genetics · 2024Article
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Authors and funding
10 authors.
Funding
Abstract
Gene drive systems could be a viable strategy to prevent pathogen transmission or suppress vector populations by propagating drive alleles with super-Mendelian inheritance. CRISPR-based homing gene drives convert wild type alleles into drive alleles in heterozygotes with Cas9 and gRNA. It is thus desirable to identify Cas9 promoters that yield high drive conversion rates, minimize the formation rate of resistance alleles in both the germline and the early embryo, and limit somatic Cas9 expression. In Drosophila, the nanos promoter avoids leaky somatic expression, but at the cost of high embryo resistance from maternally deposited Cas9. To improve drive efficiency, we test eleven Drosophila melanogaster germline promoters. Some achieve higher drive conversion efficiency with minimal embryo resistance, but none completely avoid somatic expression. However, such somatic expression often does not carry detectable fitness costs for a rescue homing drive targeting a haplolethal gene, suggesting somatic drive conversion. Supporting a 4-gRNA suppression drive, one promoter leads to a low drive equilibrium frequency due to fitness costs from somatic expression, but the other outperforms nanos, resulting in successful suppression of the cage population. Overall, these Cas9 promoters hold advantages for homing drives in Drosophila species and may possess valuable homologs in other organisms.
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