Evidence map›Paper›PMID 38809629›Full record

ArticleAsian Pacific journal of cancer prevention : APJCP2024

Momordica cochinchinensis (Gac) Aril Suppresses Proliferation and Induces Apoptosis of Colorectal Cancer Cells.

Ratsada Praphasawat, Kanathip Singsai, Phuridee Muenkaew, Sarita Sirisawat, Watanyu Jaewcharoenchai, Sinitra Thakaew, Supatthra Komkhan, Anchalee Rawangkarn

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Article in Asian Pacific journal of cancer prevention : APJCP, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Ratsada PraphasawatDepartment of Pathology, School of Medicine, University of Phayao, Thailand.
Kanathip SingsaiUnit of Excellence of Pharmacological Research on Medicinal Plants, University of Phayao, Thailand.ORCID 0000-0002-8558-9787
Phuridee MuenkaewDepartment of Pharmaceutical Care, School of Pharmaceutical Sciences, University of Phayao, Thailand.
Sarita SirisawatDepartment of Pharmaceutical Care, School of Pharmaceutical Sciences, University of Phayao, Thailand.
Watanyu JaewcharoenchaiDepartment of Pharmaceutical Care, School of Pharmaceutical Sciences, University of Phayao, Thailand.
Sinitra ThakaewDepartment of Microbiology, School of Medical Science, University of Phayao, Thailand.
Supatthra KomkhanDepartment of Microbiology, School of Medical Science, University of Phayao, Thailand.
Anchalee RawangkarnDepartment of Microbiology, School of Medical Science, University of Phayao, Thailand.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundGac aril contains high level of carotenoids. This carotenoid possesses several pharmacological properties including antioxidant, anti-inflammatory, and anti-tumor activities.

objectiveTo investigate the anti-cancer activity of Gac aril extract on human colorectal cancer cells and its related mechanisms.

methodsColorectal cancer cell lines HCT116 and HT29 were treated with Gac aril extract and its effects on cytotoxicity and anti-proliferation were analyzed using the MTT/MTS and colony formation assay, respectively. Then, further related mechanisms responsible for anti-proliferation were investigated by cell death detection ELISA and Flow cytometry.

resultsThe results showed that treated cells became rounded up and there was a loss of contact with neighboring cells, leading to a reduction of cell viability. The cytotoxic effects were evaluated IC50 for HCT116 and HT29 cells were 2.16 mg/mL and 1.29 mg/mL, respectively but it not toxic to normal HEK293 at the same dose. Moreover, Gac aril extract significantly inhibits proliferative ability with increasing concentrations having a greater effect. Subsequently, the cellular mechanism responsible for suppressive proliferation was validated. It shows apoptosis induction and arrest of cell cycle.

conclusionOur findings demonstrated that Gac aril extract can induce apoptosis and arrest of cell cycle at S and G2/M phases in both HCT116 and HT29 colorectal cancer cells.

Indexed as

ApoptosisCell ProliferationColorectal NeoplasmsMomordicaPlant ExtractsCell CycleHCT116 CellsHT29 CellsHumansTumor Cells, CulturedPlant ExtractsAnti-colony formationCell cancer arrestGac aril extractHCT116HT29

Identifiers

PMID38809629
PMCPMC11318799

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.