Evidence map›Paper›PMID 38809444›Full record

ArticleClinical oral investigations2024

A three-dimensional cell culture approach to investigate cytotoxic effects and production of inflammatory mediators by epoxy resin-based and calcium silicate-based endodontic sealer.

Miriam F Z Scelza, Sandro J O Tavares, Pantaleo Scelza, Gabriel S Ramos, Lilian Rachel de Lima Aboud, Lucila Piasecki, Paulo Emílio C Leite, Jéssica Dornelas da Silva, Sheila Coelho Soares-Lima, Gutemberg G Alves

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Article in Clinical oral investigations, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Miriam F Z ScelzaPost-graduation Program in Dentistry of Fluminense Federal University (UFF), Niterói,, RJ, Brazil. scelza@terra.com.br.
Sandro J O TavaresPost-graduation Program in Dentistry of Fluminense Federal University (UFF), Niterói,, RJ, Brazil.
Pantaleo ScelzaGeriatric Dentistry Department, Faculty of Dentistry of Federal Fluminense University (UFF), Niteroi, RJ, Brazil.
Gabriel S RamosUndergraduate of Faculty of Biology Science, Fluminense Federal University, 24210-201, RJ, Niterói, Brazil.
Lilian Rachel de Lima AboudDentistry Faculty of Université Laval, Quebec, Pavilon de Lá Terrasse, Canada.
Lucila PiaseckiDepartment of Periodontics and Endodontics, University at Buffalo, NY, USA.
Paulo Emílio C LeiteClinical Research Unit of the Antonio Pedro Hospital, Fluminense Federal University, RJ, Niterói, Brazil.
Jéssica Dornelas da SilvaClinical Research Unit of the Antonio Pedro Hospital, Fluminense Federal University, RJ, Niterói, Brazil.
Sheila Coelho Soares-LimaMolecular Carcinogenesis Program of National Cancer Institute (INCA), 20231-050, Rio de Janeiro, André Cavalcante Street, no 37, Brazil.
Gutemberg G AlvesClinical Research Unit of the Antonio Pedro Hospital, Fluminense Federal University, RJ, Niterói, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesThe aim of the present study was to assess the cytocompatibility of epoxy resin-based AH Plus Jet (Dentsply De Trey, Konstanz, Germany), Sealer Plus (MK Life, Porto Alegre, Brazil), calcium silicate-based Bio-C Sealer (Angelus, Londrina, PR, Brazil), Sealer Plus BC (MK Life) and AH Plus BC (Dentsply) through a tridimensional (3D) culture model of human osteoblast-like cells.

methodsSpheroids of MG-63 cells were produced and exposed to fresh root canal sealers extracts by 24 h, and the cytotoxicity was assessed by the Lactate Dehydrogenase assay (LDH). The distribution of dead cells within the microtissue was assessed by fluorescence microscopy, and morphological effects were investigated by histological analysis. The secreted inflammatory mediators were detected in cell supernatants through flow luminometry (XMap Luminex).

resultsCells incubated with AH Plus Jet, AH Plus BC, Sealer Plus BC and Bio-C Sealer extracts showed high rates of cell viability, while the Sealer Plus induced a significant reduction of cell viability, causing reduction on the spheroid structure. Sealer Plus and Seaker Plus BC caused alterations on 3D microtissue morphology. The AH Plus BC extract was associated with the downregulation of secretion of pro-inflammatory cytokines IL-5, IL-7, IP-10 and RANTES.

conclusionsThe new AH Plus BC calcium silicate-based endodontic sealer did not reduce cell viability in vitro, while led to the downregulation of pro-inflammatory cytokines. CLINICAL SIGNIFICANCE: Choosing the appropriate endodontic sealer is a crucial step. AH Plus BC demonstrated high cell viability and downregulation of pro-inflammatory cytokines, appearing reliable for clinical use, while Sealer Plus presented lower cytocompatibility.

Indexed as

Calcium CompoundsCell SurvivalEpoxy ResinsMaterials TestingRoot Canal Filling MaterialsSilicatesCell Culture Techniques, Three DimensionalHumansInflammation MediatorsMicroscopy, FluorescenceOsteoblastsCalcium Compoundscalcium silicateepoxy resin-based root canal sealerEpoxy ResinsInflammation MediatorsRoot Canal Filling MaterialsSilicatesCalcium silicate-basedCell viability assaysEpoxy resin-basedInflammatory mediatorsThree-Dimensional Cell Culture

Identifiers

PMID38809444

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.