Evidence map›Paper›PMID 38809040›Full record

ArticleBioconjugate chemistry2024

Comparison of Cyclic and Linear PEG Conjugates.

Grace E Kunkel, Qingyang Zhou, Joseph W Treacy, Hayden R Montgomery, Pedro Salas-Ambrosio, Austin D Ready, Alexander M Spokoyny, Kendall N Houk, Heather D Maynard

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioconjugate chemistry, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Grace E KunkelDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, United States.ORCID 0000-0001-8137-7552
Qingyang ZhouDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, United States.
Joseph W TreacyDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, United States.ORCID 0000-0002-8597-0246
Hayden R MontgomeryDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, United States.
Pedro Salas-AmbrosioDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, United States.ORCID 0000-0002-0922-4620
Austin D ReadyDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, United States.
Alexander M SpokoynyDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, United States.ORCID 0000-0002-5683-6240
Kendall N HoukDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, United States.ORCID 0000-0002-8387-5261
Heather D MaynardDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, United States.ORCID 0000-0003-3692-6289

Funding

Inorganic Chemistry Tools for Bioconjugation, Recognition and ImagingR35GM124746 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Alexander Michael Spokoyny · 2017 to 2026
$4.0M
Circular Dichroism SpectrometerS10OD028491 · OD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI LOO, JOSEPH A · 2020 to 2020
$100k
NIGMS NIH HHS R35 GM124746NIH HHS S10 OD028491
6 · The paper itself

Abstract

Bioconjugation of polymers to proteins is a method to impart improved stability and pharmacokinetic properties to biologic systems. However, the precise effects of polymer architecture on the resulting bioconjugates are not well understood. Particularly, cyclic polymers are known to possess unique features such as a decreased hydrodynamic radius when compared to their linear counterparts of the same molecular weight, but have not yet been studied. Here, we report the first bioconjugation of a cyclic polymer, poly(ethylene glycol) (PEG), to a model protein, T4 lysozyme, containing a single engineered cysteine residue (V131C). We compare the stability and activity of this conjugate with those of a linear PEG-T4 lysozyme analogue of similar molecular weight. Furthermore, we used molecular dynamics (MD) simulations to determine the behavior of the polymer-protein conjugates in solution. We introduce cyclic polymer-protein conjugates as potential candidates for the improvement of biologic therapeutics.

Indexed as

Molecular Dynamics SimulationMuramidasePolyethylene GlycolsBacteriophage T4MuramidasePolyethylene Glycols

Identifiers

PMID38809040
PMCPMC11191396

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.